Journal of Basic Research in Medical Sciences
Volume:1 Issue: 4, Autumn 2014

Sugar cane is a giant plant of grain products. Regarding the benefits of the use of medications with herbal origin, in the present study the impact of sugar cane extract on atherosclerosis and LDL-c and HDL-c in the serum of hypercholesterolemic rabbits was studied.

24 adult male New Zealand rabbits race with an average weight of 2 kg were classified into four groups: the control group had a normal diet, Sham group and the 1 , 2 experimental group were cholesteroled with 2% high cholesterol regime and received drug solvent, sugar cane extract with 3.7 and 7.4 mg/kg doses as an oral treatment per day, respectively . After eight weeks of treatment, Blood were collected and subjected for measuring of LDL-C and HDL-c. For histological studies, Aorta was removed and was fixed in formalin %10. SPSS with ANOVA was applied for data analysis.

The results demonstrated treatment via sugar cane extract with 3.7 and 7.4 mg/kg per day, significant reduction in LDL-c and significant increase in HDL-c, in compare with Sham group, was occurred. In addition, histological examination showed that treatment by 3.7 and 7.4 mg/kg per day of Sugar cane extract (the experimental groups 1, 2, respectively), prevented atheroma plaques.

Sugar cane extract may be effective in reducing the amount of LDL-c and increasing HDL-c and prevents the formation of atheroma plaques.

In vitro maturation and development of immature oocytes, as an artificial reproduction technique, is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment has not been reported yet. Present study was designed to assess the effect of heated human follicular fluid (hHFF), which is similar to in vivo environment for oocyte, on the maturation and fertilization potential of mouse immature oocytes.

Healthy female mice, aged 4-6 weeks, were sacrificed via cervical dislocation and their ovaries were extracted under sterile conditions. After washing, the separated immature oocytes were divided into three groups: In the first group, 236 immature oocytes were placed in culture medium contained DMEM, HCG, FSC 25%, and rFSH. In the second group, 229 immature oocytes were put in culture medium contained 100% hHFF. In the third group, 255 immature oocytes were placed in culture medium contained DMEM, HCG, rFSH, and 25% hHFF. Immature oocytes were placed in an incubator for 24 hours. Then, the stages of oocyte maturation were assessed by invert microscope and mature oocytes in each group were transferred to sperm-contained drops. After 24 hr, rate of two-cell embryos was recorded using invert microscope. Data was analyzed by Chi square test.

Maturation rate of oocytes in the second group (87.8%) was significantly higher than first (64.9%) and third (63.2%) groups (p<0.0005). The difference between first and third groups was not statistically significant (P<0.2). The formation rate of two-cell embryo in the second group (82.1%) was higher than first (50.2%) and third (54.3%) groups (p<0.002 and P<0.01, respectively).

it seems hHFF could improve in vitro maturation and fertility potential of immature oocytes and consequently the formation rate of two-cell embryos in mice, in comparison with DMEM even supplemented with 25% hHFF.

Job stress is one of the main characteristics of modern life which is increasing and has been recognized as an important risk factor of cardiovascular diseases. The present study was conducted to determine the association between job stress at work environment and cardiovascular diseases.

A number of 300 persons including 150 cases and 150 controls were considered in the present study. The participants were diagnosed as patient or healthy persons, based on the cardiologist visits. Then, ERI and demographic questionnaires were completed by interview with subjects. After data collection, the data was interpreted, scored and analyzed using SPSS-16 (chi-square and independent sample T tests).

Mean age of subjects was 54.2±11.3 years. There was a significant difference between case and control groups in term of extrinsic effort and esteem reward (p=0.05). No significant difference was also observed between case and control groups in term of effort and reward imbalance (p=0.059). Mean fasting blood sugar and triglycerides in patients with effort-reward imbalance was higher than in patients with effort-reward balance (p=0.05).

Cardiovascular disease has a close association with risk factors including job stress. Health education and screening of risk factors of cardiovascular diseases are recommended to early diagnosis of such diseases.

Students in elementary schools due to the physical and behavioral characteristics more than adults have exposed to environmental hazards. This study was conducted to examine the environmental health and safety indices in girls' elementary schools in Ilam city.

This cross-sectional study was carried out via census sampling method in girls' elementary schools (21 schools) in Ilam city. Data were collected through a checklist of management system evaluation. Finally, the data were analyzed by SPSS-16 and the schools were classified in 5 levels (1 to 5 stars).

The results showed that 71.4 %, 66.7 % and 85.7 % of the schools were desirable in term of providing clinical services, improving nutrition in schools and parents and society participation in health promoting program, respectively. However, 100% of the schools in term of promoting the school staff were in undesirable condition.

According to the results, most schools in term of environmental health and safety indices were in moderate condition. It was revealed that 33% of the schools, based on the health improvement program, were classified as 1 star and no school was found as 5 stars. This issue requires more attention to improve and promote the schools health level by related authorities.

Curriculum has a major impact on education. The faculty members are the most important element in the success of the curriculum. The aim of this study was to determine the viewpoints of university of medical science faculty in relation to factors affecting their participation in the academic curricula.

This was a cross-sectional study which included 66 members university of medical science faculty, from Ilam-IR, during the year 2014. Data collection tool was a questionnaire and sampling method was census.

According to findings, scientific (27.4±9.8) and outside the organization factors (9.1±2.8) on their participation in the curriculum was moderate . The impact of Professional factors (28.4±7.2), internal organizational factors (25.7±6.1) , internal (19.1±4.5) and external motivational factors (19.1±4.5) on members' participation in curriculum planning was the high level. Motivational factors most impact on the participation of faculty in academic curricula.

Organization and educational managers should use programs that can improve job satisfaction, organizational commitment, responsibility and self-confidence, improving scientific, academic freedom and professional autonomy, attention to the law, the facilities and faculty teamwork culture, workshops and conferences to increase their participation in academic curricula recommended.

Neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. Also, neurotropic factors are growth factors like Neurotrophins that help neurons survive. Moreover, Neurotrophins differentiate between progenitor cells so that neurons are formed. Despite the fact that the majority of mammalian brain neurons are produced prenatally, the capability of growing new neurons from neural stem cells will be preserved by parts of the adult brain. This process is known as neurogenesis. Neurogenesis is stimulated and controlled by neurotrophins.

The recombinant plasmid transformed to E.coli ´ cell and colonies that contain plasmid were selected by Colony PCR. Enzyme digestion and sequencing were monitored to approve the accuracy of extracted plasmid of the clones.

Plasmid was verified correctly. Based on RT-PCR and western blotting, the transcription of NTF4 gene and the expression of NTF4 protein after transfection were proved.

Plasmid was correctly constructed, CHO Cells were successfully transfected by transfection, and protein could be properly expressed. The results provided a solid foundation for the studies in the area of the transplantation of gene-modified CHO Cells to further spinal cord regeneration.

High calorie diet is becoming the most important health problem in most developed western societies. Diets with high caloric contents such as high fructose intake are associated with increasing risk of type two diabetes (T2D) and insulin resistance (IR). However, large differences have been seen in the dose and duration of fructose consumption to induction of T2D. Interest to develop animal models, in which IR is induced by feeding a high caloric diet, is the main aim of this study.

Male Sprague-Dawley rats were randomly sorted into three groups, control and fructose-treated (10 and 20 %) animals. After 6 and 14 weeks, fluid intake, body weight, intraperitoneal glucose tolerance test, metabolic parameters (glucose, insulin, triglyceride, cholesterol, uric acid) and homeostasis model assessment of insulin resistance were checked.

There was no difference between the body weight of the fructose-treated (10 and 20 %) and control groups during the experiment. Fluid intake in fructose-treated ones (10 and 20 %) was significantly higher than the control group from third week to the end of experiment. Intraperitoneal glucose tolerance test was the same for all groups in either sixth or fourteenth weeks. Fasting plasma insulin, triglyceride, uric acid and homeostasis model assessment of insulin resistance and glucose tests showed significant enhancement in fructose-treated rats (20%) only after 14 weeks.

Consumption of high fructose solution (20%) for 14 weeks could induce IR, but using of low dose of fructose (10% for 6 or 14 weeks) didn’t develop it.

Blood-borne infectious agents are one of the main anxieties of Blood Transfusion Organization in all countries. According to WHO, the blood healthy and blood safety is included ensuring and monitoring of quality and efficiency for all patients requiring blood transfusion. We at the current study intend that survey prevalence HIV Ab, HCV Ab, HBS Ag Among blood donors refer to the Blood Transfusion Organization of Ilam in 2010-2011.

The sample size was folders of 4005 subjects who entered into the study with census method. Screening test was performed for hepatitis B surface antigen, Antibodies against hepatitis C virus and HIV Ab. For confirmed positive result that obtain by screening test we use for HBV, HCV, HIV respectively.

Number (percent) of subjects with hepatitis B (HBV), hepatitis C (HCV) and HIV among the infected blood donors were 29 (70.7%), 11 (26.8%) and 1 (2.5%), respectively. There was no significant association between education level and disease (P>0.05), Significant correlation (P<0.01) was found between job variables and disease

The low prevalence of HIV infection in this study and other similar studies in Iran May be due to the higher percentage of more regular volunteer donors in Iran As the World Health Organization studies show that voluntary donors are with the lowest prevalence of HIV, hepatitis viruses and other blood-borne infections