نشریه بیولوژی کاربردی
سال یازدهم شماره 2 (پیاپی 42، تابستان 1400)

One of the types of social studies is the study of the indigenous languages of a region, which is a direct relationship with the relations of the members of a community who were in contact with each other in different eras. Since trees and shrubs have played an important role in the daily life of human societies, so has the need to use specific names for them in societies. In this study, we tried to survey different areas of Gilan, Mazandaran and Golestan provinces and conduct interviews also to study the authoritative sources of local names of trees and shrubs in the Oleaceae, Rosaceae, Salicaceae, Tiliaceae, Ulmaceae trees. In this study, 106 local names for the trees of the genus Oleaceae (10 local names), Rosaceae (43 local names), Salicaceae (25 local names), Tiliaceae (6 local names) and Ulmaceae (22 local names) in Iran, which are mainly the names used in the three provinces of Gilan, Mazandaran and Golestan in different dialects, were recorded.

Using cell culture and tissue culture techniques, many researchers are working to elucidate the hormonal factors necessary for the structural differentiation and functional activities of ovarian tissue. Therefore, in this study, we have studied ovarian tissue culture on mesenchymal stem cells (MSCs) derived from bone marrow. Hence, in this study, we examined ovarian tissue culture on mesenchymal stem cells isolated from cultured adipose tissue.

In this study, mesenchymal stem cells became isolated from adipose tissue of the mouse. After four days and monolayer formation of Mesenchymal stem cells, obtained ovarian was placed on the cultured Mesenchymal stem cells’ monolayer for 7 days.

The results of this study show that numbers of mature follicles for treat group compared to the control group were 61/2±1/2 and 38/1 ± 9/9, respectively which had a significant increase compared to control group (P<0/05). This shows the efficiency of tissue culture in co-culture with Mesenchymal stem cells. Also, which has been shown the efficiency of tissue culture in co-culturein the presence of mesenchymal stem cells. The real-time PCR studies also confirmed the decrease in the BAX genes in treatment Groups to compare the control group.

According to the results yielded regarding the number and quality of obtained follicles, it seems that this method is efficient and of high importance in producing mature follicles, and subsequently high-quality oocytes and embryo.Keywords: Tissue culture, Cell culture, Ovarian tissue, Mesenchymal stem cells, Mice.

The aim of this study was to investigate the relationship between BPA concentration and alterations of Notch1, Notch2, Notch3, Caspase-3 and Caspase-7 genes expression in the cumulus cells of infertile women with poor ovarian stimulation response (POR) following the antagonist protocol.

In this case-control study performed on 80 POR patients under 35 years of age, with 4-9 oocyte in puncture date, patients were divided into two groups. The first group consisted of 40 POR women who used plastic containers and the second group consisted of 40 POR women who used less plastic containers. Changes in the expression of Notch and Caspase genes in cumulus cells were evaluated using Q-PCR technique.

In the POR patients, the expression of Notch1-3 genes in the first group was significantly lower than the second group (p<0.05). In contrast, the expression of Caspase-3 and Caspase-7 genes in the first group was significantly higher than the second group (p<0.05).On the other hand, in both groups, was a statistically significant correlation (p<0.05) between the expression levels of Notch1-3, Caspase-3 and Caspase-7 genes and quality of Oocytes. Also, concentration of BPA in the follicular fluids of the first group was higher compared to the second group (p<0.05).

Alterations in the expression of Notch and Caspase genes in POR patients are associated with increased BPA concentration. Also, Increasing of the BPA concentration can be considered as an effective factor on the reducing of reproduction and oocyte growth.

The fagus orientalis lipesky species includes around one-third of width and correspondingly, the standing volume in the mixed and partly pure stands of northern forests of Iran.. Present research has been carried out to investigate the effects of topographic factors on quantitative characteristics of oriental beech species in Nav Asalem forestry plan. According to purpose of the research, breast height diameter and height of the oriental beech trees were measured in three altitude levels (800 to 1650 m), five slope classes (0 to 50) and four main geographical classes and recorded on the corresponding forms. The results of one-way variance analysis test showed that two factors of slope and the sea-level altitude had significant effects on quantitative characteristics of oriental beech species wherein Dunkan’s test was used to compare the means and eventually, the results showed that the lowest and highest rates of quantitative characteristics existed respectively in heights of 1300-1440m and 1440-1650m. Also, studying the slope, the highest diameter, cross-section and height of the beech trees were obtained in a slope of 40-50 percents. Thus, due to the nature and ecological characteristics of oriental beech and since the best habitats for the trees of this species to have the greatest diameter and height are the upstream and sloping regions, the results of this research show that Nav Asalem forest habitat is one of the best habitats of the target species.

From health perspective, swimming pools must have appropriate physical, chemical, and microbial properties and must be desirably maintained. In this research, physicochemical parameters, microbial, and fungal contamination of swimming pools have been examined

In this study, 11 active covered swimming pools were sampled as census. samples were, This cross-sectional study was carried out. Microbial, and fungal contamination and temperature, free residual chlorine, turbidity, oxidation and revival parameters were measured. analyzed with software SPSS V16 was used for statistical analysis. After performing descriptive analysis of data, chi-square test, Pearson correlation and ANOVA were used to determine the relationship between the variables..

The results showed that out of 27 water samples taken from the pools of Zahedan city in terms of the frequency of parasitic infection, no parasitic samples including worm eggs, trophozoites or intestinal protozoa of pathogenic and non-pathogenic intestinal protozoa were observed. Parasitic and fungal pathogens and the amount of physicochemical factors in the water of the pools were studied, which could be due to the knowledge and attitude of pool managers and managers to the importance of pool water quality to ensure the health of swimmers and monitoring to improve pool water quality (including proper chlorination).

The results indicate the It is recommended to continuously disinfect the surrounding environment including the pools platforms. The overuse of chlorine will had a negative impact on the other parameters.

An experiment was conducted to study the effect of two plant growth regulators (gibberellic acid and quintine) on seed germination and initial growth of seedlings and tolerance to salinity in two cultivars of Kimia and Shirvan. This experiment was conducted as factorial with a completely randomized design. Statistical analysis of the studied traits at germination stage and seedling growth showed that salinity reduced the rate of germination and seedling growth in both cultivars. The results of the experiment showed that the inhibitory effect of salt on seedling growth of Shirvan locality was significantly higher than that of Kimia, which in fact indicates the similarity of salinity tolerance in germination stages and complete planting in these two cultivars. Gibberellic acid increased germination speed in both cultivars, but the Kimia genotype was the highest mean for traits (root length, seedling length, root ratio with shoot). But local genotype Shirvan had the highest average in the study of traits such as (relative water content, texture water content, shoot length, number of roots).