مجله پژوهش های ژنتیک گیاهی
سال هشتم شماره 1 (بهار و تابستان 1400)

Nutrient deficiencies are important abiotic stresses that can affect plant growth and development. In this study, 76 sunflower pour lines collected from different regions of the world were evaluated in pot using some physiological traits with combined analysis of completely randomized design with three replications under optimal and phosphorus deficit conditions. Phosphorus deficiency decreased the means of all studied traits except canopy temperature. Oilseed sunflower lines were grouped into five and four clusters in each one of optimum and phosphorus deficient conditions, respectively. However, in both optimum and phosphorus deficient conditions, lines 19, 21, 27, 44 and 71 were classified into desirable cluster with high yield and yield components. Multivariate tolerance index (MFVD) for each genotype was calculated using the ratio and productivity matrices of the studied traits under optimal and phosphorus deficit conditions using principal component analysis on the resulting matrices. Based on the resulting biplot, lines 71, 74, 65, 21, 39, 7, 18 and 11 were introduced as desirable and phosphorus deficit tolerant lines.

The present study was performed to classify and study genetic diversity between Calotropis procera accessions from different regions of Kerman province (Iran) using ISSR markers. In total, DNA from 14 plant samples with nine ISSR primers was amplified by PCR and their banding pattern was obtained. The primers showed acceptable polymorphism (35.93) and minimum and maximum polymorphic information content (PIC) of primers in this study were 0.11 for ISSR9 primer and 0.41 for ISSR3 and ISSR8 primers, respectively. Genetic similarity based on Nei index was varied from 0.405 to 0.745 and the lowest genetic similarity was found between J3 (Related to Jiroft) and D2 (Related to Dosari) and the highest genetic similarity was found between J1 and J2 (both of them for Jiroft). By using UPGMA cluster analysis, samples divided into four groups, and the second and third groups contained more accessions. In terms of genetic similarity, two accessions of Jiroft 1 (J1) and Jiroft 2 (J2) which classified in the same cluster were closer. Also, the accessions collected from Anbarabad were at a longer genetic distance than other accessions. Principal coordinate analysis also showed that the first and second components justify 67 percent of obtained genetic diversity. In general, ISSR markers were useful for classifying Calotropis procera accessions and according to the obtained information about existence of genetic diversity between Calotropis procera accessions of Kerman province, this diversity could be useful in the future for breeding and production of Calotropis procera.

Due to the importance of date palm production in Iran, introduction of new cultivars, improvement of current cultivars and studying the genetic diversity among available cultivars is essential for improvement the quantity and quality of date palm production. In this research, 15 different local genotypes of date palm collected from Saravan, Jalgh, Nahook and Sinokan regions of Sistan and Baluchistan province were used for diversity analysis. To this end, DNA was extracted from leaves using Delaporta method and DNA quality and quantity were determined using spectrophotometer and 1% gel electrophoresis. PCR was performed using specific rbcL primers under determined conditions and the amplicons were sequenced. To study the relationships and genetic distances between genotypes, the results of sequencing were analyzed and dendrogram of phylogenetic relationships as well as sequence similarity matrix were generated using Bioedit, and MEGA7 software. The results of the present study showed that there were a total of 553 different residues for this marker of which 505 residues contained deletion and addition, and 48 residues were without deletion and addition. The genetic distance ranged from 0.0 to 0.037 and the highest intra-regional diversity was related to cultivar Jm13_sabzoo. Based on the dendrogram obtained from cluster analysis, the studied cultivars were divided into two branches, in which the first branch contained cultivar Sabzoo from Jalgh region and the other cultivars were grouped in different sub-branches of second branch. Although the rbcL marker is useful for studying and recognizing diversity of intraspecific relationships, a low genetic distance was estimated for the studied date palm genotypes. However, it is suggested that the other DNA barcodes as well the other appropriate molecular markers could be used for future studies of date palm genetic diversity.

Analysis of genotype by environment interaction using different statistical methods is very important in plant breeding. In order to evaluate the seed yield stability of promising sesame lines using different parametric and non-parametric statistics, an experiment was conducted using 13 promising sesame lines with check variety Oltan at three locations of Karaj, Mashhad, and Moghan (Iran) in a randomized complete block design with four replications over two years (2016 and 2017). Combined analysis of variance for seed yield of promising sesame lines showed that the effect of genotype and the three-way interaction of genotype × year × location at the level of 0.01% probability were statistically significant. Karaj-96 environment with 1346 kg/ha and Mashhad-96 environment with 1001 kg/ha had the highest and lowest mean yield, respectively. The highest and lowest mean seed yield among genotypes in all test environments were related to G6 line with 1444 kg/ha and G12 line with 762 kg/ha, respectively. Heatmap along with cluster analysis divided both genotypes and stability parameters into three groups. Based on cluster analysis, genotype G12 was clustered into the first group, genotypes G1, G3, G7, G8, and G13 were clustered into the second group and the rest of the genotypes along with the check cultivar Oltan were clustered into the third group. The genotypes of the second group with the highest rank in most criteria of stability stasistics were stable compared to other genotypes and among them, the genotypes G8, G1 and G3 (with mean yields 1417, 1398 and 1291 Kg/ha, repectively) were selected and recommended in the test locations due to their average yield above the average yield of all genotypes.

The study of the genetic structure controlling agronomic traits is one of the preconditions for selecting the appropriate breeding method. In order to analyze genetic of some agronomic traits of bread wheat using generation mean analysis, parents and different generations resulting from the cross of Marvdasht × MV-17 wheat cultivars were assessed in a randomized complete block design with three replicates under normal and terminal drought stress conditions in Research Farm of Razi University (Iran) during 2015-2016 cropping season. Based on the results of weighted ANOVA, a significant difference was observed between different generations for most of the studied traits under both conditions. Generations mean analysis revealed that in addition to the additive and dominance gene effects, a variety of epistatic effects also played a role in the inheritance of most traits, and therefore cannot hope for the success of selection in first generations. The role of additive gene effect was greater than the dominance one for plant height, peduncle length and awn length, indicating usefulness of using recurrent selection to aggregate these genes followed by selecting lines with favorable agronomic characteristics. The model of genetic control for most of the traits was similar under both conditions in terms of the presence or absence of non-allelic interactions and it has not been widely affected by drought stress. The broad-sense heritability for plant height, peduncle length and awn length was estimated to be high under both conditions. The broad-sense heritability for kernel yield was moderate estimated under both conditions, but the narrow-sense heritability was very low. In general, considering the greater role of the non-additive gene effect for most of the traits, the selection is suggested in advanced generations and after access to a high level of gene fixation.

Twenty-two G. glabra populations were used to study the genetic diversity of ISSR molecular markers. 12 primers were used to amplification of genomic DNA fragments of G. glabra populations. High genetic diversity based on ISSR markers was observed among individuals. A total of 130 bands were formed and 105 bands were polymorphic. The mean polymorphism percentage among studied populations was 80.47. The highest polymorphic percentages were assigned to IS23, IS21, IS9, IS13 and IS15 primers. The mean of PIC and MI were 0.347 and 2.47, respectively. The Shannon index (I) varied between 0.207-0.393 and the Nei genetic variation index (h) from 0.140 to 0.026. Darab and Solataniyeh populations showed the lowest and highest genetic diversity, respectively. The percentage of polymorphic loci was varied between 35.224 to 65.71%. The observe allele number and effective alleles number was 1.46 and 1.34, respectively. Based on the genetic distance Nei, populations Bardsir and Baft had the highest genetic similarity (0.888) and populations Bardsir and Solataniyeh had the least genetic similarity (0.132). The studied populations were grouped into three main groups by cluster analysis using UPGAM and Jaccardchr('39')s similarity coefficient. The results showed that the ISSR marker is a reliable marker system for revealing a high level of polymorphism and can be used to study genetic diversity and further examinations as a subset of breeding programs in G. glabra.

The challenge of the interaction of genotype × environment is one of the main issues in plant breeding. Various statistical methods to estimate the interaction of genotype × environment and choice the stable and productive genotype(s) have been introduced. In this study, 14 lentil genotypes along with two controls (Sepehr and Gachsaran cultivars) were evaluated during four growing seasons (2016-2020). The experiments were conducted in a randomized complete blocks design in three replications at Sarab Changai Agricultural Research Station, Khorammabad (Iran). The combined analysis of variance was used to investigate the interaction of genotype × environment, and results of the analysis showed significant effects for genotype, year, and genotype × environment interaction. Genotypes G5 (FLIP2014-032L) and G12 (ILL8006) were introduced based on Si(1), Si(2), and NPi(1) statistics as stable and high-yielding genotypes. Based on various non-parametric statistics, genotypes G5 (FLIP2014-032L) with a mean grain yield of 1574.68 kg.ha-1 and G12 (ILL8006) with a mean grain yield of 1333.6 kg.ha-1 were introduced as stable genotypes. The heritability rate was estimated on the plot mean basis for yield trait in four years (0.61 ± 0.18) which indicated the capability of the studied genotypes to be selected and improved for grain yield. Based on the results of cluster analysis, the genotypes were divided into three main clusters. The highest distance was observed between the second and third groups. The first cluster included highly stable genotypes.

Durum wheat (Triticum turgidum L.), like most other crops, is affected by various stresses. Therefore, cultivars that, in addition to the ability to produce higher yields, can maintain their yield potential in different years and locations are considered superior cultivars. In order to obtain high-yielding and stable genotypes of durum wheat, 16 lines with two control cultivars Dehdasht and Seymareh were evaluated in four locations of Gachsaran, Gonbad, Khorramabad and Moghan based on randomized complete block design with four replications in three cropping seasons (2013-2016). Combined analysis of variance indicated a significant effect of genotype, environment and genotype by environment interaction. Genotypes G6 and G18 had the highest and lowest grain yield, respectively. Based on parametric methods, genotypes G3, G5, G15, G13 and G16 and based on non-parametric methods, genotypes G1, G3, G4, G5, G15 and G3 were the most stable genotypes. The most stable genotypes based on the total Kang sum-rank were genotypes G15, G5, G6 and G1. The Selection index of ideal genotype (SIIG) was used to integrate all indices into one index, based on which genotypes G5 and G15 were the superior genotypes with the highest SIIG index and grain yield. Based on all indices, genotypes G5 and G15 were the most stable genotype in terms of grain yield and can be used in cultivar introduction processes.

The aim of this study was to determine the haplotype groups and identify the specific alleles associated with desirable agronomic characteristics in bread wheat. For this purpose, 42 local bread wheat genotypes belong to Iran region and nine commercial cultivars along with Chinese Spring variety (reference genotype) were cultivated in the format of augmented design and evaluated based on their 13 phenotypic traits. The results of descriptive statistics showed that awn length and day to flowering had the highest and lowest phenotypic coefficient of variation, respectively. Eight microsatellite markers were used to investigate the haplotype variation of QTLs associated with phenotypic traits located on wheat chromosomes 4B and 7D. The result showed that the genotypes were classified into 13 and 6 haplotype groups according to the allelic comparison with the reference genotype on chromosome 4B and 7D, respectively. In order to investigate the relationship between traits and markers, analysis of variance was performed based on completely randomized design with unequal numbers of replications for each marker. In general, of the 13 traits studied, there was a statistically significant linkage for eight traits and for the three traits, an allele-specific was introduced simultaneously. If the breeders are interested in genotype selection that simultaneously have three desirable characteristics such as early anthesis, semi-dwarfing and a greater number of grains per spike, they can use an allele-specific (153 bp) of Xgwm149-4B marker.

Bread’s quality depends on wheat flours quality and quantity and for the goal to be achieved, the usage of suitable wheat varieties should be considered. Present study focuses on analyzing doubled-haploid lines of wheat’s bread backing quality and the relationship between qualitative traits and glutenins reservoir proteins. In current work, traits related to bread backing quality of 30 doubled-haploid lines of wheat including their parents and two control varieties (Ehsan and Morvarid) were evaluated. SDS-PAGE test was conducted to identify total amount of protein and the relationship between seeds reservoir proteins and qualitative traits, afterward, a test was conducted to evaluate expression of genes involved in bread backing quality. Results showed that there are significant differences on evaluated traits among all wheat’s genotypes. The highest volume of Zeleny sediment were related to DH-143 and DH-159 (34 and 31 ml, respectively), the highest amount of wet gluten were attributed to DH-159 and DH-143 (77.8 and 74.85 gr, respectively), the highest amount of dry gluten were attributed to DH-159 and DH-143 (26.21 and 25.11 gr, respectively), the highest amount of water absorption percentage were attributed to DH-159 and DH-143 (51.59 and 49.74%, respectively), and the highest percentage of protein content were attributed to DH-143 and DH-159 lines (with the amount of 18.03 and 17.72% respectively). Analyzing of bread backing quality traits indicated that DH-143 and DH-159 were better than the other genotypes. SDS-PAGE test results pointed that the highest amount of seed’s protein is attributed to DH-159 and DH-143 (28.23 and 26.63 µ/gr, respectively). Based on gene expression analysis (using real-time PCR), it was indicated that lines DH-143 and DH-159 had a higher level of expressed than the control treatments for HMW-X, HMW-Y and PDIL genes. Therefore, lines DH-143 and DH-159 could be used in breeding program for optimizing bread backing quality.