مجله دانشکده پزشکی اصفهان
پیاپی 673 (هفته اول امرداد 1401)

Neonatal sepsis is an important cause of death in neonatal intensive care units where rapid diagnosis and appropriate treatment can reduce neonatal complications. Our aim is to compare. High-sensitivity C-reactive Protein (hs-CRP) and CRP (C-reactive protein) in neonates with sepsis and non-infectious neonates.

Between December 2019 and May 2020, 173 neonates of Mashhad University of Medical Sciences, including 85 neonates with positive blood culture (case group) and 88 uninfected for the control group were included in this prospective cohort study. One ml of blood was taken from all infants for hs-CRP as well as CBC (Complete blood count) samples and culture of blood, spinal fluid and urine if needed. Latex kits were used for CRP and hs-CRP and MacConkey culture medium was used for microbial culture.

The mean of hs-CRP and CRP was significantly higher in the case group compared to the control group (P > 0.05). Comparison within the group, the mean CRP in the two groups prior to, during and after infection were significantly different (P < 0.05).

The results of this study showed that hs-CRP and CRP are helpful in diagnosing late neonatal sepsis, and hs-CRP is superior to CRP in terms of specificity and positive predictive value.

Today, Glycosylated hemoglobin A (HbA1c) is used as a diagnostic criterion for diabetes and long-term monitoring of plasma glucose level. Therefore, it is essential that HbA1c be measured accurately. The aim of this study was to investigate the common HbA1c index measurement methods in laboratories in Isfahan and compare the accuracy of their results with the reference method.

In this cross-sectional study, three blood sample of a volunteer had been sent to 37 laboratories. Among them, two laboratories measured this parameter by HPLC method and were considered as a reference. For each laboratory, the coefficient of variation (CV) was calculated. One-way ANOVA and Bonferroni post hoc test were used to compare the results of different methods of measuring HbA1c with the reference method.

Comparison of the laboratories results with the reference method showed that 60% of the laboratories reported the percent of HbA1c index beyond the permitted range with an unfavorable report. The mean and standard deviation and CV index range were 2.8 and 4.4, (from 0.65 to 25.18%), respectively. Comparison of the different methods results with the reference method showed that capillary electrophoresis and immunoturbidometry and enzymatics had good accuracy. The highest accuracy was related to capillary electrophoresis. The methods with the lowest measurement accuracy were nephelometry and column chromatography, respectively.

Despite the standardization of methods for measuring HbA1c, it was noted that different results of the same blood sample was reported. In addition to the High Performance Liquid Chromatography method, the capillary electrophoresis seems to provide a more acceptable result than other methods.

The aim of this study was to evaluate the effect of nettle extract consumption and aerobic exercise on COX-1and COX-2gene expression in mice with melanoma.

In this study, 20 adult male rats were randomly divided into 4 groups: 1. control, 2. physical exercise, 3. nettle extract, 4. physical exercise + nettle extract. A week after inducing melanoma, the experimental group consumed 30 mg / kg / day of nettle ethanol extract orally for 8 weeks. The training program consisted of 30 minutes of running on a treadmill without a slope at a speed of 16 meters per minute for the first week, and one meter per minute was added every week until it reached 22 meters per minute in the eighth week. RT PCR was used to measure the expression of COX-1and COX-2 genes.

The results of the present study showed that consumption of nettle extract and aerobic exercise caused significant changes in COX-2 levels in experimental groups compared with the control group. COX-2 levels in the extract and combination groups were significantly reduced compared to the control group. Also, the results showed that COX-1 levels were significantly different between the experimental and control groups.

The results of the present study showed that the chemical compounds in nettle extract such as flavonoids, terpenes, fatty acids and phenolics may be responsible for apoptotic and anti-cancer effects. Also, endurance training caused a significant decrease in COX2 levels compared with the control group.