Archives of Clinical Infectious Diseases
Volume:17 Issue: 2, Apr 2022

The first COVID-19 case was reported in December 2019 in China. The number of infected cases increased rapidly, and COVID-19 became a public health issue worldwide. The high transmission rate and global spreading of COVID-19 caused public anxiety and may lead to unfavorable effects on psychological health.

This study evaluated the COVID-19 impact on the public anxiety, knowledge, and behavior of Iranians.

We used a web-based cross-sectional survey and collected data from 1627 volunteers. Demographic information, anxiety self-reporting, Generalized Anxiety Disorder 7-Item Scale (GAD-7), and COVID-19–related knowledge were evaluated.

Among the participants, the dominant GAD-7 score was mild, and the self-reporting level of anxiety was 5.28/10. Women and younger people reported higher anxiety than men and older groups. Further, 69.76% of participants had good knowledge, and among them, the level of education had a positive effect on knowledge, while sex and age did not have any effect. Social media and applications were the most common source of information.

Our study showed that Iranians’ anxiety was at the medium level, and their high knowledge level about COVID-19 could affect this reduction; however, we should not ignore that less anxiety makes the matter less essential.

Neisseria meningitidis and Streptococcus pneumoniae are serious causes of invasive infections associated with high mortality and morbidity worldwide, particularly meningitis. Efficient diagnostic strategies play a crucial role in the management of disease and the prevention of overtreatment. The low sensitivity and time-consuming nature of culture and gram stain methods have led to the demand for alternative methods in clinical laboratories.

This study aims to design and develop a rapid, sensitive, and cost-effective EvaGreen-based real-time PCR to simultaneously detect N. meningitidis and S. pneumoniae.

We designed and evaluated an accurate, reliable, and inexpensive approach based on EvaGreen dye real-time PCR to simultaneously detect N. meningitidis and S. pneumoniae in a single tube from cerebrospinal fluid. Melting curve analysis was used to differentiate the amplicons of each pathogen. Analytical sensitivity and specificity of the assay were conducted by reference bacterial strains genomes. Besides, in order to clinical validation we used 53 positive CSF samples and 7 negative CSF samples.

Our assay demonstrated no amplification curve with non-target microorganisms indicating 100% analytical specificity. In the EvaGreen multiplex assay, the lower limit of detection (LLD) was nine copies/reaction for N. meningitidis and 13 copies/reaction for S. pneumoniae. The clinical validation of positive CSF samples revealed 100% sensitivity and no false positives. The reproducibility and repeatability of tested replicates indicated low intra-assay and inter-assay CVs of less than 1.5%.

EvaGreen-based multiplex real-time PCR offers a rapid, affordable, and appropriate diagnostic tool to identify the main cause of bacterial meningitis.

Staphylococcus aureus can cause a variety of infectious diseases, mainly due to its ability to shift between the planktonic and the biofilm lifestyle. Biofilm infections present a serious problem in human medicine. Biofilm-associated protein (bap) and intercellular adhesin (ica) genes are involved in biofilm formation.

This study evaluated the effect of Thymus vulgaris and Cinnamomum verum essential oils on biofilm formation and expression of icaA, icaD, and bap genes in S. aureus strains.

A total of 20 strains of S. aureus were isolated from the urine of patients. The susceptibility test was performed to determine the effect of minimum inhibitory concentration (MIC) of thyme and cinnamon essential oils on strains by broth dilution method. The expression of icaA and icaD genes was determined by measuring the cognate messenger ribonucleic acid level using real-time polymerase chain reaction (PCR).

The biofilm formation of strains after treatment with the MIC of thyme essential oil was observed as 40% weak and 60% negative biofilms, and with the MIC of cinnamon essential oil as 25% strong and 75% moderate biofilms. Real-time PCR demonstrated that 60% of S. aureus strains treated with thyme and cinnamon essential oils showed a decrease in icaA gene expression. Moreover, 55% and 70% of the strains treated with thyme and cinnamon essential oils had reduced icaD gene expression, respectively. None of the strains of S. aureus had a bap gene.

The present study showed that thyme and cinnamon essential oils reduce the expression of icaA and icaD genes, and cinnamon essential oil is more effective than thyme essential oil.

Control of the COVID-19 pandemic, its treatment, and prevention of mortality and morbidity have been the main focus of researchers over the past two years. Due to disagreement on the usefulness of different corticosteroids in the treatment of COVID-19, this work compared the efficacy of dexamethasone and methylprednisolone in the treatment outcomes of intensive critical care (ICU) patients.

The present retrospective cohort study examined clinical records of 105 COVID-19 patients hospitalized in the ICUs of Firoozabadi Hospital in 2021. Clinical outcomes, including the length of hospital stay, the need for a ventilator, and mortality, were compared between patients who received either dexamethasone (DXM) or methylprednisolone (MP). Data were analyzed by SPSS V.20 software at P < 0.05 as statistical significance.

The mean ± SD ages of the patients in the DXM and MP groups were 58.82 ± 19.29 and 60.66 ± 14.17 years, respectively, without a statistically significant difference (P > 0.05). The mean duration of hospitalization was 8.14 ± 4.36 days in the DXM group and 6.80 ± 3.34 days in the MP group (P = 0.295). Also, 19 (33.3%) cases in the DXM group an, 19 (39.6%) in the MP group needed mechanical ventilation during hospitalization (P = 0.546). Finally, 30 (52.6%) patients in the DXM group and 27 (56.2%) in the MP group died.

The findings indicated no significant difference in the mean duration of hospitalization, the need for a ventilator, and mortality in COVID-19 ICU patients treated with methylprednisolone or dexamethasone. There is a need to perform meta-analyses owing to conflicting results regarding the effects of different corticosteroids on the COVID-19 course.

Prevention of surgical site infection (SSI) in hernia repair with mesh remains the main concern. Many risk factors have been proposed; however, the role of changing the glove remains to be elucidated.

This longitudinal study was conducted on adult inguinal hernia cases referred to elective repair using mesh reconstruction. Two hundred cases were enrolled and classified into two groups, including the group that surgeon and his assistants changed the glove and the group in which the gloves remained unchanged. Age, gender, surgery duration, and the side of hernia were compared between those who developed SSI and those without this complication. Moreover, the infection rate was compared between the two study groups.

One hundred and two males (51.0%) and 98 females (49.0%) with a mean age of 42.60 ± 15.79 years old were enrolled in the study. None of the variables showed a significant difference between SSI cases and non-SSI cases. Moreover, the rate of infection was not significantly different between the two study groups.

Glove changing before mesh application indicates no considerable role in reducing SSI rate; however, further studies are needed to confirm these findings. Keywords

Immunosuppression conditions are the leading risk factors for opportunistic fungal infections. The clinical manifestation is related to the site of infection. Gastrointestinal (GI) mucormycosis is a rare form of this disease, even in immunosuppressed patients.

We report rare cecum necrosis with terminal ileum perforation resulting from infiltration of mucormycosis in the cecum wall and terminal ileum mesentery in a patient with diabetes mellitus and acute myeloid leukemia (AML). During chemotherapy for AML, the patient presented diarrhea followed by constipation, severe progressive abdominal pain, and fecaloid peritonitis. Perforation of the terminal ileum was observed during the surgery. Ileostomy, right hemicolectomy, and resection of necrotic tissue were performed, and bowel mucormycosis was confirmed on histologic examination. Unfortunately, the patient expired five days after surgery.

Gastrointestinal mucormycosis is an uncommon infection with symptoms like abdominal pain, nausea, vomiting, and melena. The mortality of GI involvement is due to perforation, peritonitis, and GI bleeding.