مجله دانشکده پزشکی اصفهان
پیاپی 713 (هفته اول خرداد 1402)

The fetus is the source of many stem cells that have great potential in repairing and regenerating different tissues and can differentiate into various types of cells including osteoblast and adipocyte. However, less attention has been paid to the isolation and differentiation ability of these cells in the culture medium. Therefore, the present study aimed to determine the isolation method, proliferation, and differentiation of fetal mesenchymal stem cells (fMSCs) derived from rat fetuses.

In this experimental study, fMSCs were isolated from 15-day-old rat fetuses using trypsin enzymatic digestion and they were cultured up to 3th passage. Then, the fMSCs were characterized by flow cytometry and differentiated into osteoblast and adipocyte cells. Cell proliferation and expression of osteogenesis- and adipogenesis-related genes were investigated in differentiated cells.

Cell surface markers of fMSCs at passage 3 were positive for CD105 and CD90 and negative for CD45 and CD34. These cells exhibited a fibroblast-like morphology. These cells had calcium deposition in osteogenic differentiation and accumulation of fat droplets in adipogenic differentiation. The osteogenesis-related genes including osteonectin, ALP, BMP-6, and Runx-2, and adipogenesis-related genes such as PPARγ and CREBBP were expressed in osteogenic and adipogenic differentiation cultures, respectively.

According to the findings of the present study, it seems that mesenchymal stem cells isolated from rat fetuses have the potential to proliferate and differentiate into osteoblast or adipogenic lineages.

Considering that Alzheimer's is a destructive disease with progressive disorders and memory loss and there is no definitive treatment for it, the purpose of this research is to investigate the effect of resistance training along with the consumption of saffron extract on the expression of Bax, Bcl-2 and Caspase genes. 3-Alzheimer was induced in the hippocampal tissue of male Alzheimer's rats.

In this experimental study, 32 adult rats were randomly divided into 4 Alzheimer's groups, including control groups, aerobic exercises, aerobic exercises + saffron extract, and saffron extract. Alzheimer's was induced by injecting amyloid beta 42-1 into the hippocampus. Resistance exercises including 3 sessions per week were performed for 8 weeks. Real Time PCR method was used to determine the expression level of the target genes. The data were analyzed using one-way analysis of variance and Tom Hen's post hoc test.

The results showed that after 8 weeks of resistance training, the expression of Bax gene in the intervention groups significantly decreased compared to the control group. Also, the expression of Bcl-2 gene in the control and resistance training group and the resistance training and extract group showed a significant decrease compared to the control group. The expression of caspase3 gene in the control + resistance training group and the resistance training and extract group showed a significant decrease compared to the control group.

It seems that resistance training and consumption of saffron extract decrease the expression of genes involved in apoptosis in Alzheimer's male rats.

Menopause is associated with numerous physical, psychological and social manifestations. Adapting to it plays an important role in women's quality of life in the rest of their lives. This study was conducted with the aim of explaining women's views on adapting to menopause.

This qualitative study was conducted by content analysis method with in-depth semi-structured interview and sampling method, based on the goal of data saturation, with the participation of 30 women aged 45 to 65 in Mashhad. Data analysis was done using the Grandheim and Lundman method, and the scientific accuracy of the data and the validity of the data were checked based on Guba and Lincoln criteria.

The average age of the participants in the present study was 52 ± 4. After analyzing the data, four main categories; Assessment and understanding of the individual from the menopause period, exposure to menopause warning signs, seeking support and self-care were extracted.

Faced with menopause, women seek self-care and support by understanding and evaluating the created conditions in order to be able to adapt to the problems of menopause and the new situation created for them. The findings of this study can be used in health policy formulation. Treatment and care of menopausal women should be useful.