نشریه زراعت و فناوری زعفران
سال یازدهم شماره 1 (پیاپی 39، بهار 1402)

In order to study competition response among saffron corms, a split plot experiment was conducted on the basis of complete randomized block design with three replications in a field in Ashkezar city of Yazd province for 3 years. The main plots were irrigation with three levels (100%, 75% and 50% of ETc) and the sub plots were fertilization management methods including control (without any fertilizer or manure), applying 10 t ha-1 cow manure, 10 t ha-1 vermicompost both by spreading, 10 t ha-1 or 5 t ha-1vermicompost buried under planting corm rows, 10 l ha-1 Humaster Saffron fertilizer for 1 or 2 times after flowering during irrigation. Results showed that under moderate water stress in comparison with control, total corm number and total corm weight per plant was declined more than 30% and 17% respectively. But average corm weight was enhanced by 12%. Total effective corm weight and total effective corm number were declined more than 50% under strict water stress in comparison with control. During 3 experimental years, total effective corm number, total effective corm weight, total corm number and total corm weight were increased by 189%, 205%, 470% and 263% respectively while average corm weight, effective corm weight ratio and effective corm number ratio were declined by 38%, 17% and 48% respectively. In summary, results showed that increasing in corm weight per plants during experimental years were mostly as a result of increasing in corm numbers. Although, big corms per small corms were decreased year by year as a result of competition among corms. Under moderate stress water, saffron plant grew up each corm weight in comparison with increasing corm number per plant while under strict water stress allocated assimilates among more corms and increases corm numbers per plant.

This descriptive research was conducted as interviews using 125 questionnaires among the saffron farmers of Torbat-Heydariyeh, Iran, during 2020-2021. The aim of this study was to compare the most important agronomic operations related to saffron cultivation among three groups of farmers (ordinary, skilled and leading farmers), and to compare the methods used by the farmers with the recommendations of saffron researchers. The classification of farmers into the three mentioned groups was based on the stigma yield in relation to the field age. Before the research, the validity of the questionnaire was confirmed and its reliability was also determined using the test-retest method. The results showed that against 15% of leading farmers, none of the ordinary and skilled farmers had agricultural education. The average education of ordinary, skilled and leading farmers were 7.1, 7.5 and 10.9 years, respectively. Corm planting date of the highest number of ordinary and skilled farmers (46.2% and 35.7%, respectively) was in September, while for the leading farmers (37.5%) was in June. The average amounts of corm application by ordinary, skilled and leading farmers was 8.9, 8.9 and 10.5 ton ha-1, respectively, and the average amount recommended by researchers was 10.4 ton ha-1. All the saffron researchers recommended the corm row planting method, which is accordance with the 30, 43.1 and 44.4% procedures of ordinary, skilled and leading farmers, respectively. The minimum corm weight recommended by researchers was 8 g, which is applied by 18.7% of ordinary farmers, 25% of skilled farmers and 100% of leading farmers. Hand weeding was the most common method of weeds control among all farmers. Contrary to the recommendation of most researchers to remain saffron leaves as mulch, the leaves are mainly used as animal feed. The appropriate field age according to researcher’s recommendation was 5-6 years, while most of the farmers use the field for 7-8 years after each planting time. In general, the low level of knowledge, mass planting method (several corms in each planting point), planting of small mother corms, inappropriate planting date and long-term exploitation of the fields were the most important cases of improper field management, especially by ordinary farmers.

Saffron is one of the most valuable spices in the world and due to its high economic value and low water requirement, it is widely cultivated in eastern Iran. In this research, which took place in the research farm of the Ferdowsi University of Mashhad, Iran, in 2021-2022, the AquaCrop model to simulate the yield of the saffron plant was calibrated and validated using field measurements. Therefore, soil moisture, biomass, and plant canopy cover area were measured with a relatively high time resolution during the growing season. Pearson's correlation coefficient, root mean square, error, index of agreement and Nash–Sutcliffe index for moisture simulation were 0.87, 7.8 mm, 0.92 and 0.62 respectively, plant biomass was 0.99, 0.3 t.ha-1, 0.99, 0.98 and also 0.98 , 5%, 0.98 and 0.93 were obtained for canopy cover . The sensitive stages of the saffron plant were determined by examining the changes in daughter corms weight, biomass, and water productivity during different stages of growth in response to water stress, and a revised scenario was proposed to improve field irrigation. By applying this scenario and running the model, the amount of daughter corms weight production increased from 5.550 to 7.693 t.ha-1 and biomass from 7.204 to 9.395 t.ha-1. The water productivity value also increased from 3.50 to 3.69 kg.m-3 and 85 mm was saved in water consumption.

Agricultural saffron Crocus sativus L. is a medicinal, widely used and expensive plant and has important effective substances: crocin, picrocrocin and safranal, which gives it the characteristics of color, taste and smell. The search for new sources producing the aforementioned apocarotenoids makes the importance of research on it more obvious day by day. Since the country of Iran has diploid wild species with characteristics close to the sterile triploid cultivated species, in the present research, two species of Caspian saffron C. caspius and Zeiba C. Speciosus, along with the cultivated species, were investigated and 6 genes active in the production of the three mentioned effective substances , CCD2, ALDH, PSY, LCY, BCH, UGT74 (and a housekeeping gene TUB as an internal control gene with constant expression in all tissues) in two flower tissues, stigma and perianth (tepal) and in five stages (time) of flowering including: The stages of bud formation (immature bud), bud ready to bloom, newly opened flower, mature flower and old flower were investigated. Using of Real Time PCR, the expression ratio (fold change) to the internal control gene (with logarithm based on 2) was investigated in different genes and different and sometimes similar expression patterns were observed with the gene expression in the cultivated species in both the stigma and stigma tissues. Based on the results obtained in this study and the increase or decrease in the expression of some genes involved in the pathways of apocarotenoids production, at the same time as the growth of the stages from the bud to the senescence stage of the flower, it is expected from the three aforementioned substances or from other apocarotenoids in the metabolom of flowers, cases have been found in the non-triploid species and create approaches for the commercial use of Iranian wild saffron species and pave the way for its commercialization.

The study of the pharmacological properties of saffron and its active ingredients is important considering the clinical and health applications that it can have in humans. Diabetes is a chronic disease characterized by decreased insulin secretion due to dysfunction of beta cells in the pancreas or increased insulin resistance. The tendency to treat diabetes with herbal medicines, which have fewer side effects than chemical medicines, is spreading day by day. This study was performed for the first time to investigate the effect of aqueous extract of saffron corm on blood glucose and liver enzymes in diabetic rats. Male rats were randomly divided into healthy and sick groups without treatment and treated with doses of 100, 200, and 300 mg/kg aqueous extract of saffron corm. Streptozotocin was used to induce diabetes. The mortality rate decreased significantly after the injection of saffron corm extract. Doses of 200 and 300 mg/kg of aqueous extract of saffron corm controlled weight loss in diabetic rats so that the dose of 300 mg/kg was not significant compared to control without diabetes. The results showed that injection of aqueous extract of saffron corm significantly reduced fasting blood glucose. A dose of 300 mg/kg on day 15 after induction of diabetes caused a significant decrease in blood glucose levels. It seems that one of the mechanisms of the hypoglycemic effect of saffron extract, which increases insulin secretion from pancreatic beta cells, was due to this event. A dose of 300 mg/kg decreased hepatic enzymes AST and ALT in blood serum. The results of this research showed that the aqueous extract of saffron corm, in addition to reducing blood sugar and controlling diabetes, regulated the activity of liver enzymes.

Iran's recent years of experience show that the economy's dependence on crude oil sales results in instability of export income. Saffron is one of the important export items of the agricultural and medicinal plants sector, which in recent years has despite the increase in the area under cultivation and production, the export has not grown in line with it. Khorasan Razavi province is one of the leading provinces in the production and trade of this product. This study aims to present the multidimensional index of export development and examine the factors affecting this index regarding the saffron product in Khorasan Razavi and its trading countries in the period of 2011-2020. The necessary statistics and information were collected from 24 exporting companies active in this field in Razavi Khorasan province and 14 exporting companies in Germany, Italy, India and China by completing a questionnaire. In order to investigate the foreign trade network of saffron and the factors affecting the export development index, a three-dimensional panel model was used. The export development index was measured using the sub-indices of export performance, export management and export strategy. The results showed that the company's history, company size, marketing strategies, export standards, research and development costs, government support policy index, and government foreign diplomacy, value of domestic products, brand development and marketing mixes are among the factors affecting the development index. It is the export of medicinal plants.