نشریه علوم و فناوری بذر ایران
سال دوازدهم شماره 2 (پیاپی 30، تابستان 1402)

In order to study the effect of auxin hormone priming on seed germination indices and seedling growth of triticale under salt stress, an experiment was carried out as a factorial experiment in a completely randomized design with four replications in seed science and technology laboratory of plant production and genetics engineering department of Agriculture Sciences and Natural Resources University of Khuzestan in 2018. Experimental factors were auxin hormone priming (control (no priming), 5 μM auxin in 3 hours, 10 μM auxin in 3 hours, 20 μM auxin in 3 hours, 40 μM auxin in 3 hours, 5 μM auxin in 6 hours, 10 μM auxin in 6 hours, 20 μM auxin in 6 hours and 40 μM auxin in 6 hours) and different salinity levels (include 0, 4, 8, 12, 16 and 20 dS/m). The analysis of variance showed that the effect of priming, salinity stress and the interaction effect of them were significant on all measured traits (except germination percetage) in 1 percent probability level. The comparison of means showed that salinity stress decreased germination traits but hormone priming reduced the negative effect of salinity stress and improved different germination indices. Also, the best concentration of Auxin hormone priming was at 20 μM for 3 hours.

Salinity stress reduces seed quality, but suitable concentrations of silicon and nickel can improve germination indices in the produced seeds. This study was conducted in a factorial arrangement based on randomized complete block design (RCBD) in 2018 in Haji Abad city, Hormozgan, Iran. The experimental treatments consisted of four safflower varieties as the first factor, including Soffeh, Local Isfahan, Padideh, and Golmehr; and seven spraying levels, including nickel (100, 200 and 300 mg L-1) and silicon (100, 150, and 200 mg L-1) as the second factor. The results demonstrated that the foliar application of 100 mg/l Nickle and 200 mg/l silicon increased potassium by 10% and 18%, respectively. The foliar application of 200 mg/l silicon increased the activity of alpha and beta amylase enzymes by 17% and 12%, respectively. In addition, Golmehr and Safe varieties obtained from a mother plant recorded higher germination and rate percentage, as well as seed vigor, while Padideh variety showed the lowest values of the same traits. The foliar application of 200 mg/l silicon, compared to the control, increased the seed vigor as well as Soffeh and Golmehr cultivars by 23% and 25%, respectively; however, the Nickle concentration of 300 mg/l resulted in a decrease of 24% and 19% in these cultivars.On the other, this trait decreased with increasing nickel concentration significantly. Generally, foliar application of silicon at concentrations of 150 and 200 mg L-1 was found to improve seed quality of different safflower varieties under salinity stress.

Given the importance of salinity at the germination stage, this study was carried out with the aim of ‎investigating the diversity of germination traits in bread wheat 91 cultivars and 204 landraces along with ‎three control varieties (Sistan, Narin, and Arg) under salinity (60 and 120 mM NaCl) as a factorial ‎experiment in a completely randomized design with two replicates in petri dishes. There was a high ‎diversity between wheat genotypes in terms of shoot and root length of seeding, shoot-to-root length ‎ratio, germination index, and seed vigor under both sat stress conditions; while germination energy and ‎germination percentage had low variation. There was a positive significant correlation between all the ‎traits (except the root length of seeding and the shoot-to-root length ratio) under all three conditions ‎‎(control, 60 and 120 mM NaCl). From the factor analysis, the PC1 and PC2 under 60 mM NaCl ‎treatment were named as seedling weight and germination percentage, respectively. Under 120 mM ‎NaCl treatment, PC1 and PC2 were named as germination percentage and seedling weight, respectively. ‎Based on the biplot, IP624925, IP628189, and IP624838 landraces and ADL, AFLAK, KHAZAR1, and ‎RIJAW cultivars were identified as superior genotypes under 60 mM NaCl treatment. Moreover, ‎IP627103, IP624596, IP626566, IP625433, and IP628189 landraces and Shiroodi and Dastjerdi ‎cultivars were selected as superior genotypes under 120 mM NaCl treatment. Overall, the selected ‎genotypes were identified as genetic material with the potential of salinity tolerance based on ‎multivariate statistical analysis. ‎

In order to investigate The effect of priming and aging on the growth indicators and activity of antioxidant enzymes in hybrid maize single cross 704 a factorial experiment was conducted based on a completely randomized design at the University of Mohaghegh Ardabili in 2021 with 3 replications. Treatments were aging (control, 7 and 14 days) and priming (control, hydro-priming, priming by gibberellin (20 mg / l) and salicylic acid (100 mg / l)). The results showed that aging decreased growth indicators including Radicle Length (RL), Plumule Length (PL), Seedling Length (SL), Radicle Dry Weight (RDW), Plumule Dry Weight (PDW), Radicle Fresh Weight (RFW), Plumule Fresh Weight (PFW) Seed Length Vigor Index (SLVI) and Seed Weight Vigor Index (SLWI), but types of priming, especially priming with gibberellin improved these traits. As compared to the control, the catalase, superoxide dismutase, and glutathione reductase activities increased respectively by 73%, 63%, and 21% after gibberellin treatment and 14 days of aging. The highest amount of peroxidase enzyme activity (1.797 units mg-1 protein) was related to gibberellin priming. In general, gibberellin pretreatment increased fragile corn seeds' growth indicators and antioxidant enzymes activities, and it can be used to enhance.aging compared to the control showed an increase respectively about 73%, 63% and 21%. The highest amount of peroxidase enzyme activity (1.797 units mg-1 protein) was related to gibberellin priming. In general, using gibberellin pretreatment strengthened weak corn seeds growth indicators and antioxidant enzymes and the treatment can be used to increase weak seed vigor.

An experiment was implemented based on completely randomized design with four replications to determine standard Flixweed (Descurainia sophia) germination test condition. Flixweed seeds were placed in germinator for 14 days under 6 temperatures 10, 15, 20, 25, 20-30, 35ºC exposed to three treatments of potassium nitrate (2 grams per 1000 ml of distilled water), pre-chilling (7-10ºC for 7 days), pre-soaking (24h in distilled water), two cellulose substrates of top paper and between paper, and sands. Then, germination indexes were assessed. After comparison of three mentioned treatments (potassium nitrate, pre-chilling, and pre-soaking) with control it was demonstrated that the highest germination rate belonged to pre-chilling in 15ºC.The lowest amount of germination indices and the highest percentage of dead seeds were found in 25 and 35ºC. The best treatment to improve germination and seedling vigor was applying of potassium nitrate and optimal temperatures to reach maximum of germination was 10 and 15ºC. Higher germination was achieved when top and between paper substrate were used than sand. In total, it was suggested that it would be better for potassium nitrate to be under 10 and 15ºC when top and between paper and sand were applied, respectively to optimal condition for standard germination determination was achieved, because the highest percentage of final germination, normal seedlings, and seedling weight vigor, and also the lowest percentage of dead seeds were observed.

Identification of primings that induce secondary metabolites in order to withstand cold stress can be effective in the establishment and yield of crops. This experiment was conducted in the laboratory conditions as a completely randomized design with three replications to evaluate the effect of seed priming on cold tolerace of three genotypes and one cultivar of chickpea during germination stage in 2020. Treatments include temperatures of 5, 10, 15 and 20 ° C, genotypes of MCC505, ILC8617, MCC495 and Saral cultivar and primings included control (non-primed), hydropriming, sodium chloride, acid salicylic, sodium nitroprusside, phosphorus and potassium soluble bacteria, Full spectrum of 20 essential amino acids, potassium nitrate, zinc sulfate. Results indicated that the treatments of sodium nitroprusside, hydropriming, acid salicylic and zinc sulfate prevented the germination activity from stopping due to the decrease in temperature and among them, sodium nitroprusside treatment was able to improve germination percentage, germination rate, hydrogen peroxide and catalase under 5 ° C compared to the control by 5.7, 19, 4 and 15%, respectively. In addition, the above treatmens under 20 ° C resulted in 2 and 4.7% improvement in alpha-amylase and hydrogen peroxide, respectively. Generally, the effect of priming on cold tolerance at the germination stage showed that cold stress reduces seed germination and germination rate and has a negative effect on germination indices, enzymatic and biochemical activity. However, the use of primings, especially sodium nitroprusside, moderated the effect of cold stress and improved seed characteristics under cold stress.