نشریه هیستوبیولوژی دامپزشکی
سال هشتم شماره 2 (پیاپی 16، پاییز و زمستان 1399)

Babesia is also one of the parasites that cause major losses in the animal husbandry industry. Identifying the animals carrying Babesia is importance in controlling and preventing the disease. Microscopic observation of the protozoan parasite in the stained blood in the acute form and serological tests is helpful, but most Babesia species cannot be differentiated, and false positive and negative results are generally observed in these tests. Our purpuse was to Assessment the prevalence of Babesia parasite in Andimshek city sheep during spring and summer of 2022 and gender and age effects on it, using the molecular method of polymerase chain reactionIn the spring and summer of 2022, 100 females and 100 male apparently healthy sheep from different areas of Andimshek city were completely randomly blood sampled and sent to the laboratory. After extracting DNA from blood, polymerase chain reaction and electrophoresis was performed. Data were statistically analyzed using spss software and T-test statistical method and mean and standard deviation were calculated. The prevalence of Babesia blood parasite in the female sheeps were higher than males. Also prevalence of this blood parasite in sheep over 2 year’s old was higher than under 2 years. But the prevalence rate in males and females in different ages and different months of spring and summer was not significant. The results showed that the prevalence of Babesia blood parasite in Andimeshk city sheeps in spring and summer does not depend on sex, age and season.

Ticks are one of the most important obligate and external blood-feeding parasites of terrestrial vertebrates. This terminus can harm its host by sucking blood and transmits many pathogenic organisms that cause animal and human diseases. To carry out this study, 434 ticks were collected from 2360 sheep and 123 cows from villages around Durood city. The samples were taken to the laboratory for testing. Because the purpose of the investigation was the cephalus tick, we grouped this tick into 4 groups including male and female groups with different weights. We prepared different dilutions of fuxim and cypermethrin with normal water and exposed each group to these toxins by flotation method. The effect of poison on the larvae of Rhipicephalus sanguineus mite was investigated by the method of preparing a filter bag .Rhipicephalus female tick showed more resistance to cypermethrin poison, therefore, excessive consumption of poisons should be prevented and on the other hand, poisons should be used in combination to prevent tick resistance

Sarcocystis has 130 heteroxenous species, with different cycles and morbidity. The pathogenic species of Sarcocystis for humans are Sarcocystis hominis and Sarcocystis suihominis, in which humans are the definitive host and cattle and pigs are the intermediate hosts. Sarcocystis is a common disease between humans and animals that can be important in terms of health and economy.The purpose of this study is to investigate the level of Sarcocystis protozoan contamination in the industrial slaughterhouse of Shushtar city using the digestion method.In this study, 20 carcass samples were examined randomly and during a period of 2 months, and then, in order to take muscle samples from organs of the esophagus, diaphragm, heart, thigh, and arm were separated with tweezers, pans, and a sterile scalpel.And we labeled the livestock information on the sample and finally, we transferred the samples to the laboratory in a sterile container next to the ice and checked whether they were positive or negative for the sarcocyst parasite in the laboratory with the digestion method.The results showed the highest percentage of sarcocyst detection using a method that can confirm, and also the sensitivity and speed of sarcocyst detection with a higher level digestion method.

Breeding of horses and equines for different purposes has always been done in Iran and other parts of the world. Golestan province, especially Gonbad Kavos city, is one of the hubs of horse breeding in Iran. One of the biggest problems of keeping animals is infection with all kinds of parasitic diseases.  The purpose of this study was to investigate the prevalence of external and blood parasites of horses and its risk factors in Gonbad Kavus city. This study was carried out cross-sectionally for 6 months on 128 horses with symptoms of external and blood parasites in different areas of Gonbad Cavus was done.  First, the entire body of the animal was examined macroscopically, and after all of them, a blood sample was prepared. The results of the study showed that 100 samples (78.12%) were infected with pirplasmosis blood parasite and there was no other type of blood parasite infection.  generally, the results of the present study showed that the contamination with blood parasites in Kavus Dome is high and worrying and has no relation to age and gender. The level of external parasites shows the high health of horses in the region.

Salmonella is one of the most important and common infectious diseases of humans and animals that is caused by Salmonella bacteria and causes the death of many people from human and animal populations annually. Salmonella-infected poultry meat is the primary cause of infection transmission, so identifying the prevalence of Salmonella infection in poultry flocks is a necessary step in taking appropriate control measures to prevent the transmission of infection to human communities. For random sampling, 100 chicken livers were purchased from supermarkets in Malayer city, then sent to the laboratory to evaluate the level of Salmonella infection.After performing bacterial tests, Salmonella serotype was determined using PCR method.In the present study, it was found that 2% of chicken liver was infected with Salmonella bacteria. It should be noted that 2% of the samples had bacterial contamination. It was also found that the predominant salmonella serotype in chicken livers of Malayert city is Salmonella enteritidis. According to the results of the present study, it can be concluded that food of animal origin is a good source for the growth of Salmonella in the country and it is recommended that to prevent bacterial contamination, strict health measures to prevent Contamination of water and food should be done and other livestock products should be cooked enough.

In order to investigate the effect of protoscolex hydatid cyst on the production of cysts in rabbits, from May 1401 to Tanime Khordad, a total of 25 sheep hydatid cyst samples, including 10 lung cyst samples and 15 liver cyst samples, were collected from Durood slaughterhouse and 3 ml of cyst fluid were collected under sterile conditions. aspirated and after centrifugation at 1500 rpm for 5 minutes, we poured the protoscolexes into a 2 ml Eppendorf tube. The viability of protoscolex was determined with 1% eosin dye with a light microscope. 2 groups of 4 female (group one) and male (group two) rabbits were tested. One sample from each group was not prescribed any drug or protoscolex as a control. Three rabbits from one group were injected intraperitoneally with one milliliter of cyst fluid containing about 2000 live protoscolex. The same amount of lung cyst was administered to three others from group two. After 45 days, the rabbits were killed with ether and autopsied, and cysts were isolated in two cases on the liver and peritoneal area. The size of the cyst in one female rabbit included a liver cyst of 12 mm in length and 7.8 mm in width, and in another rabbit, an abdominal cyst of 8.5 mm in length and 2.7 in width was seen. but they lacked liquid and protoscolex, so it seems that the protoscolex hydatid cyst of sheep has the ability to produce cysts in laboratory animals.