فهرست مطالب

Journal of Pathobiology Reaearch
Volume:11 Issue: 3, 2008

تاریخ انتشار: 1387/10/11
تعداد عناوین: 11

فارسی
English

انتخاب همه

The changes of αv, β3, α4, β1 integrins expression and osteopontin their ligands in murine estrous cycle

Fatemeh Peyghambari, Mojtaba Rezazadeh Valujerdi, Mojdeh Salehnia*, Mehdi Forouzandeh Pages 1-7

Objective
Considering the importance of integrin molecules in the implantation and lack of sufficient information in the expression pattern of these molecules in various phases of estrous cycle. It seemed to be necessary to investigate these molecules in mouse endometrial during the various phases of oestrous cycle.
Materials And Methods
Female NMRI mice (n=15) aged 6-8 weeks were studied. Various phases of estrous cycle including: proestrus, estrus, metestrus and diestrus were determined by vaginal smear. The mice were sacrificed (at least 3 per each phase) by cervical dislocation and the tissues were obtained from the middle 1/3 part of their uterine horns at each phase then the cryosections at thicknesses between 8-10 μ were obtained. Then the immunohistochemistry were done for integrins of 4, 1, v, 3 and their ligand osteopontine.
Results
The integrins were expressed only in the metestrous phase of oestrous cycle in the different locations of mouse endometrium. The positive reactions were observed for αv, α4 and β3 in the apical and basal membrane of glandular epithelium. Also the positive reaction for β1 was found in surface and glandular epithelium as well as stroma. The osteopontin expression was seen in the apical membranes of surface and glandular epithelium and was not seen in other locations. Conclousion: It seems that expression of integrins in endometrium is based on their role in the implantation, therefore the molecules α4, β1 and OPN that are expressed on the surface epithelial may be involve in the adhesion of cell to cell and integrins of αv, β3 that are expressed in the glandular

Abstract View Paper Original: Persian
Study the effects of static magnetic field on cell cycle progression in mesenchymal bone marrow stem cells of rat

Amir Sabet Sarvestani, Parviz Abdolmaleki*, Seyed Javad Mowla, Faezeh Ghanati, Zeinab Tavassoli, Emran Heshmati Pages 9-18

Objective
The environmental exposure to Magnetic Fields (MFs) may interact with biological systems. MFs are generated from various sources such as power lines, electric appliances at homes and offices, electrified transportation systems including urban railway systems and diagnostic devices such as Magnetic Resonance Imaging (MRI). There are some scientific evidences that imply the exposure to MFs are hazardous to our health and increases the rate of some cancers like leukemia. The biological consequences of exposure to MFs have been investigated from a variety of endpoints. However, most studies have been performed in vitro and have examined effects on cellular processes and its malfunction; such studies can be used as evidence of effects in vivo.
Materials And Methods
In this study Bone Marrow Stem Cells were grown in the absence and in the presence of a 15 mT Static Magnetic Field for 5 hours in order to determine any changes in cell cycle progression using the count of cells in different phases. The count of cells in a special phase of cell cycle indicates the length of that phase. The Static Magnetic Field was performed using a locally designed MF generator.
Results
A significant increase in the number of cells in G0/G1 was observed in comparison with the controls. Also the number of cells in G0/G1 in the cells treated with Hydrogen-Peroxide, as an oxidative agent, was significantly increased in Static MF.
Conclusion
Genetic material damages or mal-function of related proteins may cause these halts. Mfs have not enough energy to affect the biological molecules directly but the mechanism of free radical mediators is probable. These kinds of damages (direct or indirect) can permanently bring the cell cycle to a halt.

Abstract View Paper Original: Persian
Cell cycle progression epithelium and stroma involve in the invasion of embryo; Mesenchymal Stem Cells; Static Magnetic Field

Oghol Niaz Jorjani, Fatemeh Ghafarifar*, Zohreh Sharifi, Abdolhossein Dalimi, Zahir Mohammad Hassan Pages 19-30

Objective
Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which in the infected host is obligate intracellular parasite. LACK gene is conserved among related Leishmania species. LACK is the immuno-dominant antigen of L.major which is considered as the most promising molecule for a recombinant or DNA vaccine against leishmaniasis.
Materials And Methods
In this study the genomic DNA of an Iranian standard strain of Leishmania major (MRHO/IR/75/ER) was ertracted and the LACK gene was amplilified by PCR. Then the PCR product was cloned into pTZ57R/T cloning vector, The PT-LACK recombinant plasmid was extracted from transformed E.coli bacteria (TG1 strain) and sequenced.
Results
The LACK gene (Accession no LmjF28.2740) of MRHO/IR/75/ER & L. major was amplified using PCR method. LACK gene was cloned into pTZ57R/T coloning vector. Sequence analysis of the cloned LACK gene showed high homology 89% with LmjF28.2740 (LACK gene).
Conclusion
The LACK gene of L.major was cloned in pTZ57R/T vector successfully. Recombinant plasmid was confirmed and could be used for the production of recomiomort antigens is DNA vaccines, for further studies.

Abstract View Paper Original: Persian
Detection of unknown deletions in alpha globin genes in alpha thalassemia carriers using Real-time PCR

Somayeh Jamali, Reza Mahdian, Mina Hayat Nosaeid, Sadegh Babashah, Fereshteh Maryami, Morteza Karimipoor, Behnaz Zarbakhsh, Faezeh Rahimi Nejad, Sirous Zeinali* Pages 31-39

Objective
Alpha-thalassemia is one of the most prevalent hemoglobin disorders in the world and it is a common hereditary condition caused by deletion of one or more α-globin genes. Common α-thalassemia deletions like 3.7 kb, 4.2 kb, 20.5 kb and Med can be detected by Multiplex PCR. There are, however, some unknown deletions that can not be detected by the mentioned method or even by direct DNA sequencing. In the present study, Real-time PCR was used to determine the presence or absence of unknown deletions.
Materials And Methods
Real-time PCR was performed using intercalating dye SYBR Green I and α1, α2 and CLCN7 genes were amplified. Data analysis was conducted using comparative threshold method (ΔΔCT) for determination of Gene dosage of α1-globin and α2-globin genes.
Results
The results showed the ratio of 0.90±0.16 for normal individuals and the ratio of 0.32±0.15 for carrier samples with deletions. In addition, Melting curve analysis confirmed the specific amplification of target genes.
Conclusion
The Real-time PCR assay is simple, rapid, and reliable. It can be applied for direct determination of unknown deletions in Alpha-thalassemia carriers.

Abstract View Paper Original: Persian
A study on single nucleotide polymorphisms in Plasmodium falciparum chloroquine resistance during two years in Chabahar

Pages 41-48

Objective
Plasmodium falciparum chloroquine resistance is a major problem in malaria endemic areas. Single nucleotide polymorphisms in pfcrt and pfmdr1 genes are known to be associated with chloroquine resistance in some parts of the world. The major goal of the present study was to detect the five single nucleotide polymorphisms in pfmdr1 gene and one single nucleotide polymorphisms in pfcrt gene.
Materials And Methods
Total of 26 blood samples were collected from falciparum malaria infectious person with chloroquine failure in Chabahar, a harbor located in Sistan baluchestan during 2 years. Detection of single nucleotide polymorphisms were carried out by Real-Time PCR using Light CyclerTM hybridization probe assay.
Results
Our data showed that the pfmdr1 N86Y mutation was detected in 6(23%) samples. Although this mutation was not observed in the first year but in the second year it was substancial. In addition the pfcrt K76T mutation was detected in 11 samples (42.3%) of CVMNT haplotype, 7 samples (26.9%) of CVIET haplotype, 5 samples (19.2%) of SVMNT haplotype and 2 samples (7.6%) of SVIET haplotype.
Conclusion
The mutations considerably have increased during 2 years. Our results showed single nucleotide polymorphisms in pfmdr1 and pfcrt genes. This could be considered as chloroquine resistance markers for malaria control in Chabahar.

Abstract View Paper Original: Persian
Assessing the levels of cAMP response element binding protein1 (CREB1) after siRNA mediated knockdown in K562 cells

Pages 49-55

Objective
CREB1 is an important downstream protein for many signaling pathways. By designing efficient siRNAs against CREB1, it may be possible to assess the role of molecules involved in signaling pathways in different cell types. In this research the efficiency of CREB1 knockdown by two different siRNAs in K562 cells has been studied.
Materials And Methods
siRNAs have been designed according to the criteria suggested by Reynolds et al. K562 cells were transfected by siRNA using Lipofectamine 2000. The efficiency of CREB knockdown has been assessed by quantitative relative Real-time PCR.
Results
Our results have shown that only one of the siRNAs has a high level of inhibitory effect on CREB1 gene expression. The expression of CREB1 by this siRNA was knocked-down by 87% in K562 cells.
Conclusion
In this research, although two siRNAs were designed according to the Reynolds et al. criteria, only one showed an inhibitory effect. Reasons other than the aforementioned criteria may be involved in effectiveness of siRNAs.

Abstract View Paper Original: Persian
Development of ELISA test for determining the antibody titer against Herpes Simplex Virus-1 and comparison of the results by VNT

Pages 57-63

Objective
Herpes simplex virus (HSVs) is a widespread human infectious agent, responsible for persistent and latent infections. Herpes simplex virus infections are usually continually recurrent in the normal population and represent a significant cause of complications in immunocompromised patients.
Materials And Methods
In this study HSVs were propagated in BK cells and more than 502 samples were taken and analyzed for HSV IgG antibodies using Virus Neutralization Test (VNT) as golden standard test for evaluating in house Enzyme Linked Immunosorbent Assay (ELISA).
Results
Based on the results 80.48 % and 81.67% were positive (1.8) in VNT and ELISA respectively. There was a significant correlation between the VNT and ELISA tests in the tested samples (Pearson’s r = 0.96).
Conclusions
Our data showed that the in house ELISA can be used for screening and determination of the prevalence of HSV IgG antibodies, which can facilitates patient management using suitable and cost effective laboratory diagnostic tests.

Abstract View Paper Original: Persian
Parasitological study on Lymnaea palustris and its ecological survey by GIS in Mazandaran province

Pages 65-71

Objective
Lymnaea palustris was previously found in Mazandaran province but there was not any report about its parasitologic aspects. This study was conducted to finding ecological and Parasitological aspects of snail in Mazandaran province, North of Iran.
Materials And Methods
In this descriptive study, more than 181 locations, were checked, in 36 locations, colonies of the snail were found and 490 snails were collected. After diagnosis of snails as Lymanea palustris, in laboratory, they were crushed and their probable cercaria was checked out by a dissecting microscope. Data were analyzed and processed by ArcGIS 9.2 and Microsoft Office 2003 for descriptive analysis.
Results
from 490 snails, 6 cases (1.22%) were infected with trematode larval stage. These cercariae were classified as echinostomaercaria. Optimum temperature for the snails was 15-19 degrees of celsius and optimum dissolved salt (TDS) was 200-400 ppm. Population of colonies were raised in autumn and winter but infected snails were seen in summer.
Conclusion
This study could show the ecological pattern, distribution, and population dynamic of the snail. Also the existence of echinostomaercaria which is cercaria, generally belong to the Echinostoma sp, indicates veterinary and parasitological importance of local snails. It is probable these parasite, infect man also. More studies on definitive host and exact species of parasite are proposed.

Abstract View Paper Original: Persian

علمی مصوب

Journal of Pathobiology Reaearch

نشریه پژوهش های آسیب شناسی زیستی

فصلنامه پزشکی

آخرین شماره | آرشیو

ISSN: 2538-3000

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دانشگاه تربیت مدرس

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فهرست مطالب

Journal of Pathobiology Reaearch Volume:11 Issue: 3, 2008

Journal of Pathobiology Reaearch

نشریه پژوهش های آسیب شناسی زیستی

Journal of Pathobiology Reaearch
Volume:11 Issue: 3, 2008