مجله تحقیقات دامپزشکی
سال شصت و چهارم شماره 2 (تابستان 1388)

Progestagens have been used in order to control follide wave dynamics in camelids, however, the results are contra versial.The purpose of this study was to investigate the effect of single norgestomet implant on ovarian follicle development in Bactrian camel. Bactrian camels (n=8; 6-14 years old) were assigned randomly to two groups. Norgestomet treated group (n=4) received one implant of norgestomet under the skin of ear for 10 days (Day 0= Day of implant insertion). Camels in Control group (n=4) did not receive any treatment. Daily ultrasound examination using trans-rectal linear probe was conducted to determine ovarian follicle status. Mature follicle was defined as a growing follicle at the size of 13-17 mm in diameter. In both groups, there was a female with a persistent follicle throughout the experiment and a new emergence of follicle wave on Day 6-7 of the experiment. The latter follicle did not reach mature size on Day 10 of experiment. In both groups, there was a female with growing follicle during the experiment that became at the range of mature size at the termination of study. In both groups, there was a female with a regressing follicle throughout the experiment and a growing follicle which become mature during 10 days of treatment. One female in Control group had a persistent follicle throughout the experiment and a new emergence on Day 9. One female in Norgestomet treated group, had a persistent follicle during experiment and a growing follicle that ovulated spontaneously between Days 9 and 10 of experiment. In conclusion, single Norgestomet implant for 10 days does not regulate ovarian follicle development in Bactrian camel.

In order to determine the changes in serum and blood factors of rainbow trout following experimental infection with a pathogenic Aeromonas hydrophila, the cause of haemorrhagic septicemia in fish, three groups of rainbow trout (10 fish in each group) were injected intraperitoneally with 105, 106 and 107 of this bacteria respectively. The same number of fish in a control group were injected similarly with physiologic saline. After the appearance of clinical symptoms of haemorrhagic septicemia in fish, samples from skin mucus and blood of the fish were collected in order to determine lysozyme, anti- Aeromonas hydrophila immunoglobulins (measured by plate method and ELISA respectively), total white and red blood cells(WBC/RBC). Results showed that the lysozyme level in skin mucus and sera was significantely higher than the control group (p<0.05) while the immunoglobulin level of skin mucus of experimentally challenged fish was not significantely higher than that of control group except that of the group 2 and 3 of the experimental fish. WBC/RBC and hematocrit of the experimentally challenged rainbow trout were significantely higher than the control. Thus infection of rainbow trout with Aeromonas hydrophila increases the level of mucus and serum lysozyme, serum immunoglobulins, decreases RBC count and heamatocrit percentage.