نشریه تحقیقات دامپزشکی و فرآورده های بیولوژیک
سال بیست و پنجم شماره 1 (پیاپی 94، بهار 1391)

In this study brown algae، Sargassum glaucescens and that found plenteously in Persian Gulf and Bushehr coast، were collected and hot water extracts of them were lyophilized. F. indicus (11. 32±1. 20 g)، after two weeks adaptation in Shoghab research station dependent to Iran Shrimp Research Center، ISRC، were immersed in seawater (39 ppt and 25±1 C○) containing hot-water extract of brown algae، S. glaucescens، at 100، 300 and 500 mg/l concentration، prevention of White Spot Disease and immunological parameters (total haemocyte count (THC)، total plasma protein (TPP) and Phagocytic activity) were examined. According to results، immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours significantly enhanced THC، TPP and Phagocytic activity. Immersion in seawater containing 100، 300 and 500 mg/l hot-water extract of S. glaucescens after 3 hours، improved the survival rate of WSSV-infected F. indicus.

American foulbrood disease (AFB) is one of the most important bacterial diseases of honey bee can be caused severe losses in apiaries. AFB caused by Paenibacillus larvae a Gram positive and spore-forming bacterium. This study was taken placed in 15 area of east Azerbaijan province of IRAN between 2006-2007 years. In this study، 500 bee hives were randomly selected from 100 apiaries. Standard Bacteriological tests was performed to isolate Paenibacillus larvae Identification and Antibiogram tests were done on approved isolates. The results of this study revealed that، three apiaries were infected by Paenibacillus larvae and they were sensitive to the Oxytetracycline، Tetracycline، Doxycycline، Sulphadimidine، Taylosin، Gentamicin، Streptomycin and Penicillin respectively. Finally، it was demonstrated that the rate of AFB in apiaries of east Azerbaijan province is low and isolated bacteria were sensitive to the most antibiotics.

In this research Psedomonas fluorescens as a probiotic، sodium chloride، plant extract (Terminalia catappa)، malachite green and formalin as antifungal agents were used daily for 19 days on rainbow trout fertilized eggs in order to compare their survival with untreated fertilized eggs in control group. Results showed that the percent of live eggs (eyed) after 19 days treatment with P. fluorescens (107 bacteria/ml)، sodium chloride (20000 ppm)، the plant extract (5 ppm)، malachite green (66. 7 ppm)، formalin (200 ppm) and the control group were 81. 3%، 86. 7%، 82. 9%،83. 4%، 89. 2%، and 77. 2% respectively. Thus P. fluorescens as a probiotic، the plant extract and the other anti-fungal agents revealed significant effect in rainbow trout fertilized eggs survival anti-fungal activity as compared with the control group (P<0. 05). The plant extracted antifungal agent and also the probiotic as a new agent used in this study compete with the commonly used antifungal in that these are environmentally friendly non-hazardous agents.

Sheep is one of the important hosts for hydatidosis in Iran as a hyperendemic region worldwide. In the present study، the efficacy of counter immuno-electerophoresis (CIE) and PCR tests to detect sheep hydatid cyst infection was carried out. For this، soluble antigen was provided using hydatid cyst fluids (HCFs). Blood sera were taken from a total number of 100 slaughtered sheep. CIE analysis was undertaken using negative and positive controls. DNA was extracted from protoscolices and a 1213bp fragment length of co1 gene amplified. Necropsy findings indicated that lung and liver of slaughtered sheep (33%) was infected with hydatid cyst. While it was 29% in CIE and 28% in PCR test. According to the necropsy findings as a gold standard test، sensitivity and specificity of CIE was 79. 31% and 92. 2%، respectively. Sensitivity and specifity of PCR test were determined 85. 19% and 94. 8%، as well. Data analysis showed that there is no significant difference between these two techniques (P<0. 05). Based on these results، CIE technique can be considered as a useful method to screen sheep hydatid cyst infection.

This Survey was Conducted on 133 egge that they were collected from Isphahan markets، Eggs are tested for aerobic mesophill bacteria (Total count) and 186 eggs were tested for salmonella spp. contamination. The results showed 3000 to 10 million colony per gram bacteria counted in egg samples. The mean colony count was 1693000. 7 percentage samples rich to mean colony count. (95% accuracy،SD2. 232) the total count rate were 68 percentage under average and 25 percentage upper average. Salmonella entritidis was isolated from 3eggs samples. among 3 cases salmonella confirmed in 2 cases by PCR.

A survey on endo-parasitic infections on Barboid fishes of South- West of Iran (Khouzestan province) was carried out from spring 2007 to atumn 2009. Altogether 296 specimens from five different fish species were examined، they are Barbus grypus Heckel، 1843; Barbus sharpeyi Günther، 1874; Barbus esocinus Heckel، 1843; Barbus barbulus Heckel، 1849 and Barbus pectoralis Heckel 1843. The fishes were collected from five stations of Karoun River (Ahvaz-Goulestan، Molasani، Dez Dam)، Karkheh River (Hamidyeh Dam) and Shadgan Lagoon. The fishes transported alive to the laboratory where they measured and weighted. Then they were killed by cutting the spinal cord. According to the results of this study eight different species of endo-parasites were revealed، from which five of them identified to the species level and three to the genus level. The metazoans are as follows: Two digeneans: Diplostomum spathaceum Rudolphi، 1819; Asymphilodora sp. Looss، 1899; Five nematods: Rhabdochona denudata Dujardin، 1845; Philometra karuensis Pazooki & Molnar، 1998، Philometra sp.، Pseudocapilaria tomentosa Dujardin، 1843Contracaecum larvae sp. Railliet & Henry، 1912 and only one Acanthocephalus: Neoechinorhynchus tylosori. According the results of this study one new host; Barbus pectoralis for Diplostomum spathaceum form Iranian freshwater fishes was introduced and Neoechinorhynchus tylosori for the first time was recorded from Barbus barbulus from Iran.

Botulism is a zoonotic disease which has a worldwide distribution. The source of infection in livestock is usually carcasses of birds، domestic and wild animals. Rotten carcasses of animals are contaminated by Clostridium botulinum. The toxin of this bacteria leads to a fatal paralytic disease. This paper reports an outbreak of domestic animal botulism in the villages of Bushehr province in 2006. Intestinal contents of 10 cows، 15 goats and 12 sheeps that showed clinical signs were examined for presence of botulism’s toxin according to guideline of control disease center. Tests on autopsy samples and intestinal contents indicated the presence of botulinum toxin in affected animals and in autopsy studies rat carcass were found in for stomach of animals. To prevent botulism in drought conditions، modifying dietary deficiencies by use of phosphorus and protein supplements is recommended and sanitary landfill of carcasses is recommended to prevent contamination of other pastures.