مجله دانشکده پزشکی اصفهان
پیاپی 247 (هفته اول مهر 1392)

Human growth hormone (hGH) is a secretory protein of the blood which is used extensively in biotechnology and medical sciences. Recombinant human growth hormone production is still one of the priorities of the Iranian Ministry of Health. One of the most sensitive and accurate techniques for the measurement of this hormone is the ELISA method. Scientists need to produce antibody against hGH to measure the serum level of hGH in patients. Commercial ELISA kits are commonly imported from other countries to Iran. Therefore، the current study aimed to produce a polyclonal antibody against hGH in order to make a domestic ELISA kit to measure this hormone. Rabbit polyclonal antibody against hGH was produced following 10 weeks of immunization by the antigen injection. The antibody characteristics were evaluated by immunoblotting. The purified antibody was used to design the ELISA kit. The designed sandwich ELISA method was standardized concerning the amount of antibody coating and antibody conjugate. Ultimately، the sensitivity and specificity of the designed kit was compared with the foreign kits. The polyclonal antibody against hGH was produced in rabbits and purified successfully. This antibody can be used in the western blot and ELISA methods. The diagnostic value of the designed kit was 98%، compared with the foreign kits. This kit has a diagnostic value in the assessment of hGH; therefore، it can be a good alternative to the commercial kits. Therefore، this antibody and ELISA kit can be produced more cost-effectively and can be a step towards self-sufficiency.

Human growth hormone (hGH) is a secretory protein of the blood which is used extensively in biotechnology and medical sciences. Recombinant human growth hormone production is still one of the priorities of the Iranian Ministry of Health. One of the most sensitive and accurate techniques for the measurement of this hormone is the ELISA method. Scientists need to produce antibody against hGH to measure the serum level of hGH in patients. Commercial ELISA kits are commonly imported from other countries to Iran. Therefore، the current study aimed to produce a polyclonal antibody against hGH in order to make a domestic ELISA kit to measure this hormone.

Rabbit polyclonal antibody against hGH was produced following 10 weeks of immunization by the antigen injection. The antibody characteristics were evaluated by immunoblotting. The purified antibody was used to design the ELISA kit. The designed sandwich ELISA method was standardized concerning the amount of antibody coating and antibody conjugate. Ultimately، the sensitivity and specificity of the designed kit was compared with the foreign kits.

The polyclonal antibody against hGH was produced in rabbits and purified successfully. This antibody can be used in the western blot and ELISA methods. The diagnostic value of the designed kit was 98%، compared with the foreign kits.

This kit has a diagnostic value in the assessment of hGH; therefore، it can be a good alternative to the commercial kits. Therefore، this antibody and ELISA kit can be produced more cost-effectively and can be a step towards self-sufficiency.

One of the most prevalent intestinal cancers is rectum cancer causing 10% of mortality rate due to cancer. According to relative high prevalence of rectum cancer in Isfahan، Iran، the aim of this study was determining survival time and recurrent rate of this cancer in this region. This cross-sectional study for survival analysis was done in Alzahra hospital (Isfahan، Iran) during 2012-2013. 101 hospitalized consecutive patients with rectum cancer were selected. The necessary data such as demographic information، pathologic results and etc. were achieved from patients'' records. We contacted patients'' families for achieving completing data. All patients were followed for prognosis، death and recurrent after the surgery. The mean of survival time in all patients was 5. 45 ± 0. 45 years. According to Kaplan-Meier analysis، the median survival was 6. 0 ± 0. 9 years. Mean and median survival was statistically different based on the disease stage (P < 0. 001); but other factors had not positive effect on patients'' survival. The mean and median of relapse were 14. 05 ± 2. 30 and 12. 00 ± 0. 83 months، respectively. Chem-radiotherapy before the surgery statistically affected relapse time but other factors were not effective. Because of the high relapse and low survival rate in our patients in comparison with world statistics، protocols of treatment for rectum cancer must be revised.

Obstructive Sleep Apnea (OSA) is a type of sleep related breathing disorder due to upper airway obstruction with the high risk of 26% among adults. According to some researches، it seems there is a relation between OSA and epilepsy. Therefore، this study aimed to evaluate the risk of this disorder and daytime sleepiness in patients with complex partial seizure. This analytical descriptive study was done on 90 patients referred to Alzahra Neurology Clinic in Isfahan، Iran، in 2012. They were divided into two case and control groups (n = 45). The first consisted of Complex partial seizures (CPS) patients and the second was non-epileptic patients. Sleep Quality Questionnaire was completed for the participants، and demographic information، type and doses of used drugs، and seizure recurrence cases in the previous 3 months were gathered for each patient. Data were analyzed by SPSS software. The mean value of OSA in the case and control groups were 1. 95 ± 1. 41 and 1. 84 ± 1. 43، respectively، with no significant difference (P = 0. 71). The mean score of EPWORTH questionnaire were 5. 1 ± 4. 4 and 5. 3 ± 4. 8 in the two groups; which were not significantly different. There was no evidence of OSA association with epilepsy. However، due to the importance of OSA it is recommended that further studies be carried out in this regard. Moreover، it is necessary for epileptic patients to gain definitive diagnosis. Then، the effects of OSA on improvement of seizure control، and their quality of life must be studied.

Obesity and Diabetes are two metabolic diseases that are dependent on genetic and various environmental factors. Today، obesity and type 2 diabetes cannot just be attributed to genome changes، bad eating habits، or physical activity reduction. Gut microflora is another effective factor that has an important role in health. Recently، numerous experiments have been done to investigate the relation between metabolic diseases and bacterial populations in the gut. The purpose of this study is to assess the bifidobacteria of the intestinal microbiota in people with type 2 diabetes and compare with healthy persons. In this study، 40 male and female adults، 20 of whom were diagnosed with type 2 diabetes، were studied. For this study، age، gender، and body-mass index (BMI) are matched in diabetic and healthy persons. After fecal sample collection from diabetic and healthy persons، and extraction of DNA from samples، culture and extraction of DNA from standard bifidobacteria were done as a positive control. After that، cloning in PTZ57 R/T plasmid and extraction cloning plasmid was done. For quantitative investigation of bifidobacteria، Real-time PCR molecular technique was done by using special primer of bifidobacterium and standard curve. To study the number of bifidobacteria in people with type 2 diabetes and compare with healthy persons، statistical analysis was done. Average number of bacteria in people with type 2 diabetes is more than in healthy persons، but Student’s independent t-test) P = 0. 09) and Mann-Whitney test (P = 0. 1) indicate that there are no meaningful differences between them. Quantitative calculation with Real-time PCR indicates that the number of bifidobacteria is not effective in Iranian type 2 diabetes patients.