javad sayyahi

Today, with increasing resistance to synthetic drugs, the demand for natural and safe alternatives to these drugs increases. Therefore, the aim of this study was to determine the antibacterial effect of ethanolic extracts of Ziziphus Jujuba, Medicago sativa, Reum ribes and Hyssopus officinalis on some standard bacteria.

This experimental study was performed in the Microbiology Laboratory of Islamic Azad University, Ahar Branch. Plants were identified by botanists after being collected from natural habitats. Extraction was performed by Soxhlet extractor and the antimicrobial effects of the extracts were determined by agar well diffusion and determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) on standard bacteria in vitro.

Z. Jujuba and M. sativa plant extracts have been effective on gram-negative bacteria and extracts of R. ribes and H. officinalis plants have been effective on gram-positive bacteria, with increasing the concentration of extracts, the antibacterial property increases significantly. MBC / MIC results showed that Z. Jujuba and M. sativa plant extracts were most susceptible to P. aeruginosa and E. coli and R. ribes and H. officinalis extracts were most susceptible to S. aureus and B. cereus.

Z. Jujuba, M. sativa, R. ribes and H. officinalis plants had significant antibacterial effects, but to introduce these plants as antibacterial drugs requires more complete and comprehensive studies in vivo.

The incidence of drug resistance against chemical antimicrobial drugs, has led in recent years to the use of medicinal plants in the treatment of infections is high. The aim of this study was to determine the antibacterial effect of plants Centaurea depressa M.B., Reseda lutea and Fumaria asepala is on some pathogenic bacteria.

plants of the city of Marand East Azerbaijan province of Iran in the spring were collected by botanists and Agriculture Organization, C.depressa M.B., R.lutea and F.asepala and medicinal plants were approved. After preparation of the plant extracts, concentration concentrations 50- 400 mg / ml, the extract from the agar well diffusion and disk diffusion method on bacteria were studied. The minimum bactericidal concentration and the minimum inhibitory concentration (MIC / MBC) were performed by dilution test.

The results showed that the inhibitory effect of alcoholic extracts of C.depressa MB and R.lutea the Gram-positive bacteria, Gram-negative bacteria is more than alcoholic extract of F.asepala The inhibitory effect on gram-negative bacteria than bacteria Gram positive. Analysis of test MBC / MIC showed that extracts of medicinal plants most susceptible to B.cereus and E. coli was the least sensitive.

the plant extracts tested in vitro on the growth of pathogenic bacteria has significant inhibitory effects. In order to further clinical trials are necessary clinical application of these extracts.

As much as people become aware of the dangerous side effects of synthetic antibiotics, the demand for natural alternatives to these drugs increases. Natural ingredients, lower risk of complications and even have beneficial side effects. The aim of this study was to determine the antibacterial effect of herbs Reum ribes L and hyssop Hyssopus officinalis is on some pathogenic bacteria.

After collecting and confirming the scientific name, the methanolic extract of R. ribes L. and  H. officinalis  plants was prepared and the antimicrobial effects of the extracts by agar well diffusion and disk diffusion , as well as the determination of The minimum bactericidal concentration and the minimum inhibitory concentration (MIC / MBC)  were dilution test on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa.

The highest growth inhibitory zone in S. aureus, B. cereus, P. aeruginosa, and E. coli at concentrations of 400 mg / ml, respectively, in the disc method of 13.21, 13.41, 11.2 and10.74 mm and the well method, respectively 13.64, 13.11, 10.67 mm, and 9.38 mm for the R. ribes L extract, and the disc method of 11.74, 10.2, 10.71, and 9.1 mm, and the well method of 12.41, 11.6, 10.2, and 9.9 respectively. 4.3 mm was observed for H. officinalis extract. The results of MBC / MIC showed that the extract of medicinal plants had the highest susceptibility to B. cereus bacteria and the least susceptibility to E. coli.

R. ribes L. and  H. officinalis  plants have significant inhibitory effects on the growth of pathogenic bacteria in vitro. Therefore, it can be expected that these extracts can be used for the treatment of bacterial infections and are a good alternative to the usual chemical treatments for the treatment of infections.

Nowadays, the prevalence of drug resistance to synthetic drugs is increasing in many countries in the world; therefore, many efforts have been made to find new compounds as an appropriate alternative to antibiotics. This study was performed to investigate the antibacterial effect of methanolic extract of Carum copticum L. on some pathogenic bacteria.
Methods and Materials: In this descriptive-laboratory study, after collecting the plants and confirming its scientific name by botanists, methanolic extract of the plant was prepared by Soxhlet extractor method and different concentrations of extract were prepared. Then, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) extracts were determined on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa using well-diffusion and dilution methods.
The results showed that MIC and MBC of methanol extract on Staphylococcus aureus, Bacillus cereus and Escherichia coli were 12.5, 25 and 50 mg / ml, respectively.
Discussion and The results of this study show that Carum copticum L. plant can be considered in herbals groups with antibacterial properties. After evaluating their effects in vivo condition and identifying the active ingredients, it can be used as an alternative to synthetic drugs that commonly used to treat infections.

Nowadays, development of drug resistance against chemical antimicrobial drugs has attracted attention using medicinal plants in treatment of infections. The aim of this study is to evaluate the antimicrobial effects of two species of Centaurea plant’s essential oil on drug resistant clinical isolates of three pathogenic isolates.
The studied plants were collected from Marand city in East Azerbaijan, Iran and were confirmed as Centaurea Depressa M.B. and Centaurea Cyanus L. by botanists of Iran Agriculture Organization. The essential oil of these plants (Stems and leaf) were extracted via steam distillation method by Clevenger, and their antimicrobial effects were studied by well diffusion method in the abovementioned bacteria. The components of essential oil were identified by injection to gas chromatography linked to mass spectrometry (GC/M).
The results of this study prove that the essential oils from the abovementioned plants have bactericidal effects. The most antibacterial effect is observed in Escherichia coli strains. The results of GC/MS chromatography reveal that the essential oils of Centaurea Depressa M.B. and Centaurea Cyanus L. have 28 and 32 compounds, respectively.
This study confirmed that the grasses could be used in medicinal plants group with antibacterial properties. However, their effects in vivo must be evaluated and the most effective component of them must be identified carefully so that they can be applied commonly as an alternative synthetic drug in treating infections.