mohammad sookhtanloo

Cardiovascular diseases are the leading cause of mortality worldwide. Despite the importance of early diagnosis and advances in medicine, one-third of Myocardial Infarction (MI) cases remain undiagnosed. Biomarkers are a reflection of the physiologic function and pathologic changes in the body. Saliva is a refined plasma that can replace blood to facilitate and accelerate the diagnosis process given the benefits of its collection and storage. The present study aimed to investigate the correlation between serum and saliva hs-CRP and Irisin concentration in MI patients.

In this case-control study, 46 cases were selected including 24 acute MI and 22 controls. The serum and saliva samples were collected and the amount of Irisin and hs-CRP was measured in each sample in the specialized biochemistry laboratory of Mashhad Medical School. Data were analyzed using SPSS (version 22) by Pearson correlation coefficient test (P<0.05).

The concentration of Saliva Irisin was significantly lower in the patient group than that in the control group (P=0.021). The levels of serum and salivary hs-CRP were significantly higher in the patient group than those in the control group (for both P<0.001). The concentration of marker in saliva significantly increased as the concentration of marker increased in serum (P<0.001).

Measurement and reduction of salivary Irisin concentrations can be a reliable criterion for diagnosing and predicting MI. The concentration of hs-CRP in saliva and serum is directly related to MI.

Periodontitis is an inflammatory disease which affects vascular supporting tissues of teeth. Angiogenesis is an integral component in the pathogenesis of vascular diseases and chronic inflammations, such as periodontitis. On the other hand, one of the major regulators of new vessels formation is vascular endothelial growth factor (VEGF). The aim of this study was to determine and compare VEGF levels in gingival crevicular fluids (GCF) among patients with aggressive and chronic periodontitis and healthy individuals.
In this cross-sectional clinical study, forty-five volunteers were divided into three equal groups consisting of healthy individuals, chronic periodontitis and aggressive periodontitis. In order to perform sampling, GCF was absorbed by putting number 40 endodontic paper cones in the the gingival sulci with highest degrees of clinical attachment loss and inflammation signs. Similarly, mesiobuccal sulci of left maxillary first molars were sampled in healthy individuals. After collecting all samples, VEGF levels were determined using ELISA method.
The present study showed that VEGF concentration means in healthy individuals, chronic periodontitis and aggressive periodontitis were respectively 192.63±170.07, 1409.38±244.12 and 1734.33±317.13 nanograms per liter. VEGF levels in patients with aggressive periodontitis were significantly higher than chronic periodontitis (P=0.003) and VEGF levels in patients with both types of periodontitis were significantly higher than healthy individuals (P0.05).
VEGF levels were significantly higher in both types of periodontitis than healthy individuals and were significantly higher in aggressive periodontitis than chronic periodontitis.

Achillea is a plant widely used in traditional medicine for gastrointestinal disorders. The aim of this study was to investigate the preventive effect of Achillea wilhelmsii hydro-alcoholic extract on indomethacine-induced gastric ulcer in rats. It was an interventional study and done on six groups of male Wistar rats (n=8 in each group). Three doses of Achillea wilhelmsii extract (200, 400 and 800 mg/kg) were given orally to three groups for three days. After the third day the indomethacine in methyl cellulose (1%) suspension were given orally to the rats and the animals were anesthetized after 6 hours and the pylorus ligatated for one hour. Then the gastric juice extracted and gastric acid titrated by NaOH. The preventive ratio, gastric mucus secretion, NP-SH compounds and lipid peroxidation were measured. Achillea wilhelmsii extract clearly prevented indomethacine- induced ulcer. The Achillea wilhelmsii extract reduced gastric acid secretion by 400 and 800 mg/kg doses compare to the indomethacine group. The extract increased gastric mucus which was significant by 800 mg/kg dose. NP-SH compounds increased significantly by 200 and 800 mg/kg doses in comparison the indomethacine group. Lipid peroxidation noticeably reduced by all three doses. The Achillea wilhelmsii extract showed obviously preventive effect on indomethacine-induced gastric ulcer. Although the potency of the extract on inhibition of gastric acid secretion and increase of gastric mucus secretion are important in this preventive effect but antioxidant effect of the extract is more prominent.