mohsen amini

Organic acids remarkably affect the microbial control, stability, and organoleptic characteristics (flavor, color and aroma) of beverages. This study focuses on the determination of organic acids, including oxalic, citric, tartaric, malic, succinic, lactic, fumaric, acetic, propionic, and gallic acid, in 100 commercial malt beverages from different brands (five Iranian and five various imported brands) and flavored variants (classic, pomegranate, peach, tropical and lemon). In addition, the contents of total phenols, total flavonoids, ascorbic acid, and free amino acids were measured to assess the overall composition. Liquid Chromatography (LC) was employed to develop a method for analyzing the organic acids, while spectrophotometric techniques were used for quantifying other bioactive compounds. The results revealed significant variations in the organic acid profiles, with succinic acid being the most abundant, while tartaric acid was absent in all samples. Chemometrics technique (PCA method) was applied to classify the results. The results show that PCA can classify the malt drinks based on the additive values with a very high precision. To improve the quality control of malt beverages, some extra assessments, like organic acids and free amino nitrogen determination, should be considered for Iranian National Standard.

In this study, a rapid, simple, and advanced reverse phase high-performance liquid chromatography (RP-HPLC) method was developed for the quantification of sulfasalazine in human plasma.

Sulfasalazine was extracted from plasma matrices using a simple protein precipitation method by acetonitrile. Chromatographic conditions were optimized (mobile phase compositon, flow rate, injection volume and temperature of the oven). The method was validated in protein precipitated human plasma for linearity, selectivity, accuracy, precision, limit of detection, limit of quantification.

The chromatographic separation was conducted on C18 brisa LC2 column (250 mm × 4.6 mm, 5μ) using isocratic elusion with mobile phase consisting of the mixture of acetonitrile: 10mM Ammonium acetate pH adjusted to 4.6 (30:70 v/v) with a flow rate of 1.0 ml/min at ambient temperature. Detection was carried out by UV detector at 361 nm. Calibration curves made in the human plasma were linear in the range of 3.125-50 μg/ml with the value of r2 > 0.9999. The LOD and LOQ was 0.5 and 2.5 µg/ml, repectively.

The developed and validated HPLC-UV method is suitable for accurately determining sulfasalazine levels in pharmacokinetic studies of new formulations.

 Neuroinflammation and oxidative stress are critical factors involved in the pathogenesis of Parkinson's disease (PD), the second most common progressive neurodegenerative disease. Additionally, lipid peroxidation end products contribute to inflammatory responses by activating pro-inflammatory genes. Lipid peroxidation occurs as a result of either the overproduction of intracellular reactive oxygen species (ROS) or the reaction of cyclooxygenases (COXs).

 In this study, we examined the role of 1,5-diaryl pyrrole derivatives against the neurotoxic effects of 6-hydroxydopamine (6-OHDA) in a cellular model of PD.

 PC12 cells were pre-treated with compounds 2-(4-chlorophenyl)-5-methyl-1-(4-(trifluoromethoxy)phenyl)-1H-pyrrole (A), 2-(4-chlorophenyl)-1-(4-methoxyphenyl)-5-methyl-1H-pyrrole (B), and 1-(2-chlorophenyl)-2-(4-chlorophenyl)-5-methyl-1H-pyrrole (C), respectively, 24 h before exposure to 6-OHDA. We conducted various assays, including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), ROS, and lipid peroxidation assays, Hoechst staining, Annexin V/PI, Western blotting analysis, and prostaglandin E2 (PGE2) determination, to assess the neuroprotective effects of pyrrole derivatives on 6-OHDA-induced neurotoxicity.

 Our results demonstrated that apoptosis induction was inhibited by controlling the lipid peroxidation process in the in vitro model following treatment with compounds A, B, and, somehow, C. Furthermore, compounds A and C likely act by suppressing the COX-2/PGE2 pathway, a mechanism not attributed to compound B.

 These findings suggest that the novel synthetic pyrrolic derivatives may be considered promising neuroprotective agents that could potentially prevent the progression of PD.

Today, the economic structure of the world is fundamentally different from the past. Sustainable development today is based on innovation and creativity and the use of knowledge. Entrepreneurship development in the country, while providing the ground for sustainable growth and development, will solve current issues and problems such as poverty, social and cultural disorder, unemployment of university graduates and the massive problem of other unemployed. Therefore, due to the problems of business and most important of them, the lack of an appropriate and systematic model, a model for planning and solving the necessary problems related to activities in the food industry should be designed and used to meet the conditions and the possibility of private sector entry with minimum Risk, be provided. This research uses grounded-theory research method to explain and develop a conceptual model in the field of developing a sustainable entrepreneurship management model in the country's food industry. The model is based on ideas from the opinions and views of experts, professors and faculty members. The results of examining the opinions of experts indicate that in order to achieve sustainable entrepreneurship in the food industry (phenomenon-oriented), formulate strategies and policies, use of entrepreneurial management training mechanisms, attention to meritocracy and elite participation, standardization and regulation, regulation, regulation And writing regulations and trust, support, organizational commitment, etc. is of great importance, which leads to consequences such as increasing sustainable growth, international identification, fighting corruption, reforming related laws, and ultimately the development of the food industry and social welfare. Finally,

Fire safety is one of the most important issues in high-rise buildings. The purpose of this study is to assess maximum possible loss in the fire in Central Insurance Building of Islamic Republic of Iran using the layer of protection analysis (LOPA) method.

In 2017, this analytical study was conducted on the 21-floor building of the Central Insurance in Iran. To identify the hazard sources and assess the maximum possible loss, the authors used the preliminary hazard list (PHL) and layer of protection analysis (LOPA) respectively. In addition, the analysis of the study data was performed based on the 10×10 risk assessment matrix.

The results of the PHL showed that 26 hazardous conditions and four major sources including structural engineering, fire alarm systems and fire extinguishers, design and maintenance of building safety, and behavioral habits were identified as main hazards of fire in the studied building. The application of the LOPA method showed that highest risk level was associated with the fire caused by the exhaust heat from the engine room (RL=48) and the emergency power generator diesel (RL=40), respectively.

The findings of this study indicated that calculating the maximum possible loss in the fire of high-rise buildings can help to increase the safety factor. Moreover, the use of the two methods, PHL and LOPA, can be useful in these types of risk assessments.

The purpose of this article is to examine the position of Charles-Henri de Fouchécour (1925-AD), in Iranian studies, in his translation of Divan e Hafez. He has authored four major works in classical Persian literature: Moralia, The description of nature in 11th century Persian lyric poetry, inventory and analysis of themes, Hafez of Shiraz and Elements for a Persian textbook. Fouchécour's works (with the exception of the translation of Divan e Hafez) have not been able to make a turning point in the field of Iranian studies and Persian literature due to the reasons that we will present, most especially because they lack a comprehensive theory. Despite this, in the translation of Divan e Hafez, Fouchécour has achieved an important position in the field of Iranian studies. This translation, is a valuable work due to the translator's correct understanding of Hafez, the correct translation of the sonnets and the description of all the verses. This translation has been well received, based on the numerous reprints that have been made over the course of a decade. As a result, Fouchécour, as an Iranian Studies scholar, has a dual position. On the one hand, he has not achieved much success in his research works; on the other hand, he has immortalized his name in French Iranian Studies by translating Divan e Hafez.

Proteolysis-targeting chimera (PROTAC) is a bifunctional molecule comprising a ligand to recognize the targeted protein to be degraded.

To use the advantages of the PROTAC technique, we have synthesized novel compounds to degrade inosine monophosphate dehydrogenase (IMPDH) by the proteasome system.

We describe the synthesis of new PROTACs based on a combination of mycophenolic acid (MPA) as the potent IMPDH inhibitor and pomalidomide as a ligand of E3 ubiquitin ligase via linkers formed from Cu(I)-catalyzed cycloaddition reaction.

All synthesized compounds were investigated against Jurkat cells as acute T-cell leukemia and were potent apoptosis inducers at 50 nM.

The effect of compound 2 in 0.05 μM on IMPDH degradation can be almost prevented by competition with bortezomib as the proteasome inhibitor at 0.1 and 0.5 μM.

In this research, a reversed phase high performance liquid chromatographic method was developed for the simultaneous determination of two tyrosine kinase inhibitors, nilotinib and sorafenib. Separation was performed on an Agilent C18 column (4.6×250 mm, 5µm) with mobile phase composition of potassium dihydrogen phosphate buffer (25 mM, pH 4.2) and acetonitrile (35:65 v/v) at 1.2 mL/min with UV detection at 265 nm. Specificity, linearity, precision, accuracy, and robustness of the proposed method were all assessed. Nilotinib and sorafenib had estimated retention times of 5.1 and 5.9 minutes, respectively. Linear concentration ranges for nilotinib and sorafenib, were determined as 0.05-1 µg/mL and 10-45 µg/mL with comparable coefficient correlations (0.999). For nilotinib and sorafenib, the limits of detection (LOD) were determined as 0.030 and 0.020 µg/mL, while the limits of quantification (LOQ) were 0.101 and 0.069 µg/mL respectively.

 Hyperglycemia is a metabolic disorder that refers to an increase in blood sugar in diabetic patients. α-Glucosidase has been introduced as a membrane-bound enzyme, and it is the main enzyme for carbohydrate digestion in some parts of the intestine. Inhibition of α -glucosidase enzyme activity is a reliable approach to control post-prandial hyperglycemia condition.

In this study, a series of Pyrazolo[1,2-b]phthalazine-5,10-dione derivatives 5a–t were synthesized via a multicomponent reaction and evaluated as new inhibitors for α-glucosidase.

 The biological activity of the synthesized compounds was studied using a source of the α-glucosidase enzyme (EC3.2.1.20, Saccharomyces cerevisiae) at 20 U/mg concentration. 

 Four compounds showed higher α-glucosidase inhibitory activity in comparison to a standard, i.e., Acarbose. Compound 5q displays the most potent α-glucosidase inhibitory activity (IC50 = 155.4 ± 6.0 μM). 

In conclusion, some of the synthesized compounds, including heterocyclic core molecules, have shown remarkable activity that could be considered as subjects for the development of new, more efficient inhibitors of the α-glucosidase enzyme.

Sustainable entrepreneurship based on value creation in the context of investment is one of the most important issues for today's competitive businesses. Therefore, while paying attention to investing in this field, the concepts of value creation should be considered as very influential characteristics on it as well as the consequence of investing. To conduct this research, first, a comprehensive review of sustainable entrepreneurship management models was conducted. After the interviews, the interviews were coded; challenges, gaps and ideas for resolving them were presented. Using a systematic approach and distributing a questionnaire, the opinions of experts on the importance of sustainable entrepreneurship indicators were examined. Finally, according to Pareto's optimal law, the factors with the highest score (20% higher) were identified as influential factors. Findings indicate that individual input variables are factors of control centre, creativity, individual independence and the need for success and risk, respectively. Environmental input variables are the most important effective factors, respectively, market situation, social corruption and unemployment rate, entrepreneurship education and culture, government support and creating a legal basis. Also, the most important factors related to system outputs are the creation of industries and businesses, economic growth and sustainable development, unemployment rates and social corruption, respectively. Finally, practical suggestions were expressed for activists and researchers in the field of entrepreneurship.

Vinegar, as a traditional fermented product, plays a significant role in human health and nutrition. This product is produced in different types industrially or domestically. Under production, the vinegar may contain ethanol that its inclusion is banned in the foods by Islam and its content should be adapted to the legislations developed in Islamic countries. Therefore, at this study we measured ethanol concentration of vinegars distributed in Tehran (capital city of Iran).

Headspace-gas chromatograph equipped with flame ionization detector was developed for determination of ethanol. Acetonitrile and nitrogen were used as internal standard and carrier gas, respectively. For analysis, 140 samples (37 domestic and 103 industrial vinegars) were purchased from local market.

Our developed method could successfully determine the ethanol content at low concentration. In this regard, LOD, LOQ, and recovery were 0.0069% v/v, 0.021% v/v, and 100.14%, respectively. In 13 samples, the ethanol content was higher than 0.5% v/v that is the maximum limit determined by Iranian national standard. Out of 13 samples, 10 vinegars were domestically prepared. Evaluation of our results revealed that ethanol concentration in domestic vinegars was higher than industrial products. Therefore, domestic production of vinegars should be controlled and monitored strictly.

Prognosis of metastatic breast cancer is very poor which urges the necessity to develop novel potential drug candidates. We assessed two compounds with tri-aryl structures (A and B) for their potency to reduce primary breast tumor growth and lung metastasis in 4T1 mice model. MTT assay, 4T1 mammary mouse model and immunohistochemistry experiments were used in this study. In vitro results exhibited an anti–proliferative effect for compounds A and B towards MDA-MB-231 cancer cells. Our in vivo results displayed that administered compounds A and B could suppress the size of the primary tumor and the number of lung metastatic foci in 4T1 BALB/c mice model. Histopathological analysis revealed that both compounds treatment resulted in necrosis. Our findings provide new evidence that compound B may be promising for slowing the growth of tumor along with metastatic foci.

Somatostatin receptor-positive neuroendocrine tumors have been targeted using various peptide analogs radiolabeled with therapeutic radionuclides for years. The better biomedical properties of radioantagonists as higher tumor uptake make these radioligands more attractive than agonists for somatostatin receptor-targeted radionuclide therapy. In this study, we tried to evaluate the efficiency of Luthetium-177 (177Lu) radiolabeled DOTA-Peptide 2 (177Lu-DOTA-Peptide 2) as a new radioantagonist in HT-29 human colorectal cancer in vitro and in vivo. DOTA conjugated antagonistic peptide with the sequence of p-Cl-Phe-Cyclo(D-Cys-L-BzThi-D-Aph-Lys-Thr-Cys)-D-Tyr-NH2 (DOTA-Peptide 2) was labeled with 177Lu. In vitro assays (saturation binding assay and internalization test) and animal biodistribution were performed in human colon adenocarcinoma cells (HT-29) and HT-29 tumor-bearing nude mice. 177Lu-DOTA-Peptide 2 showed high stability in acetate buffer and human plasma (>97%). Antagonistic property of 177Lu-DOTA-Peptide 2 was confirmed by low internalization in HT-29 cells (<5%). The desired dissociation constant (Kd =11.14 nM) and effective tumor uptake (10.89 percentage of injected dose per gram of tumor) showed high binding affinity of 177Lu-DOTA-Peptide 2 to somatostatin receptors.  177Lu-DOTA-Peptide 2 demonstrated selective and high binding affinity to somatostatin receptors overexpressed on the surface of HT-29 cancer cells, which could make this radiopeptide suitable for somatostatin receptor-targeted radionuclide therapy.

Various types of alcohol for severalapplications are available worldwide, of which, disinfection is one of the most important. In Muslim nations, consumption of alcoholic beverages is prohibited even at low quantities in accordance to halal status. Therefore, denatonium benzoate (commercially known as Bitrex) that has sharp bitterness is added to alcohol to avoid its edible usage. In this regard, at least 10 mg l-1 of denatonium benzoate is added to industrial alcohol according to Iranian Ministry of Health regulation. In our study, we examined the concentration of denatonium benzoate and also purity of alcohol samples collected from capital city of Iran (Tehran).

In total, 62 samples of alcohol were collected and analyzed by HPLC for Bitrex and alcoholmeter for purity. For HPLC, C18 column (150×4.6 mm, 5 µm) as stationary phase and phosphate buffer/acetonitrile solution containing sodium lauryl sulfate (50:50 v v-1) as mobile phase with flow rate of 1.2 ml min-1 were used.

The results revealed that some companies (41 samples out of 62) did not use denatonium benzoate in their products and used fruit essences instead to improve the taste and smell of alcohol. These results were against the force of Ministry of Health in mandatory addition of denatonium benzoate to prevent the samples’ further abuse. In addition, purity of most alcohols was not compatible to the information provided by the labels. We concluded that more restriction and supervision is required to prevent adulteration.

Superwarfarin toxicity may be a serious problem. It needs high clinical suspicious in patients with bleeding diathesis without hematologic or liver diseases even in patients with apparent negative history of warfarin or other anticoagulant accessibility. Here we reported a patient with a negative history of any medical diseases or drug administration who was referred with generalized ecchymosis. Increased international normalized ratio and decreased vitamin K‑dependent coagulation factors were detected in this patient. His hematologic and liver evaluations were normal. Clinical pharmacist emphasis in taking history revealed using anticoagulant rodenticide all over the farm the patient lived in that might result in unaware intoxication in this patient who suffered dementia.

Vitamin D deficiency causes osteoporosis, osteopenia, fractures, rickets and more recently is linked with some chronic illnesses such as cancer. Because of the safety and probiotic properties of the yeast Saccharomyces cerevisiae, we hypothesized that yeast cells enriched with cholecalciferol (vitamin D3) could represent a solution for prevention or treatment of vitamin D deficiency. In this study S. cerevisiae was used as a vitamin D3 accumulator for the first time and the optimal conditions for enrichment of S. cerevisiae was determined. The Plackett-Burman screening studies were used for selection of the most important factors affecting cholecalciferol entrapment. Response surface methodology was employed for optimization of cholecalciferol accumulation in S. cerevisiae cells by using Box-Behnken design. A modified quadratic polynomial model fit the data appropriately. The optimal points of variables to maximize the response were cholecalciferol initial concentration of 358021.16 IU/ml, tryptone concentration of 1.82 g/l, sucrose concentration of 7.13 % (w/v) and shaking speed of 140.46 rpm. The maximum amount of cholecalciferol in dry cell weight of S. cerevisiae was 4428.11 IU/g. The cholecalciferol entrapment in yeast biomass increased about two-folds in optimized condition which indicates efficiency of optimization.

Oliveria decumbens is an aromatic plant traditionally used for treatment of infections and gastrointestinal diseases. In the present study, the volatile oil of the plant was obtained by hydrodistillation and analyzed by GC-MS. In addition, antibacterial and anti-Helicobacter pylori activities of this essential oil were determined using disc diffusion and agar dilution methods, respectively. Insecticidal activity was assessed through topical and fumigation application of the essential oil to cabbage looper larvae. Acetylcholinesterase (AChE) inhibition by the essential oil was examined using Ellman’s method. Furthermore, its cytotoxic potential against three different cancer cell lines was assessed using the MTT assay. The phenolic monoterpenoids, thymol (38.79%) and carvacrol (36.3%) were identified as major constituents of the essential oil. We observed significant antibacterial activity of the essential oil against H. pylori (MIC=20.4 µg /mL) as well as other tested bacteria, except for Pseudomonas aeruginosa. O. decumbens essential oil showed significant toxicity to cabbage looper larvae with LD50 value of 52.1 µg /larva following topical and fumigant administration. O. decumbens essential oil was considerably inhibitory to acetylcholinesterase activity (IC50=0.117 µg/ml). Cytotoxic assay of the volatile oil resulted in IC50 =0.065, 0.104 and 0.141 μg/mL for MCF-7, T47D and MDA- MB-231 cell lines, respectively. According to our data, this species with high concentrations of thymol and carvacrol could be considered as a natural source for pharmaceutical products.

Traumatic Brain Injury (TBI) is generally recognized as a major risk factor for memory impairments and Alzheimer’s disease (AD). In this experimental study, our aim was to investigate the ameliorating effects of luteolin (LUT) on the memory impairments, oxidative stress, and histopathological changes induced by TBI in rats. The adult male Wistar rats were randomly divided into six groups including: Control (Co), sham, TBI, TBI+LUT (10 mg/kg), TBI +LUT (25 mg/kg), TBI +LUT (50 mg/kg). To evaluate the protective effects of LUT on the memory of the rats, passive avoidance test using shuttle box was performed. Finally, the animals were anesthetized, and the brain tissues were removed and analyzed for oxidative stress parameters. Using histological methods, dark neuron production was also evaluated. There was a significant decrease in the latency time to enter the dark compartment in passive avoidance test in TBI animals. This latency time was significantly increased in TBI+LUT (25 mg/kg) and TBI+LUT (50 mg/kg) groups along with significant increases in superoxide dismutase and catalase activity in the hippocampal zone and a decrease in malondialdehyde (MDA). The number of dark neurons in the hippocampus decreased with all three doses of LUT. In the present study, LUT showed neuroprotective effects, improvement in learning and reduction in memory impairment induced by TBI in rats. Protection against oxidative stress might be a possible mechanism behind these effects. Further works are necessary to work out if LUT is potentially a suitable therapeutic candidate for neural disorders.

The basic chemical structure of most prostate specific membrane antigen (PSMA) inhibitors which are now in pre-clinical and clinical studies is Glu-Ureido-based peptides. Synthesis of urea-based PSMA inhibitors includes two steps: 1- isocyanate intermediate formation and 2- urea bond formation. In current methods, isocyanate is formed in liquid phase and then reacts with amine existing in liquid phase or bound to solid phase for urea bond formation. In this study, we developed a new facile method for formation of both isocyanate and urea on solid phase under standard peptide coupling conditions. The solid phase-bound isocyanate served as intermediate to form urea bond. To monitor reaction progress qualitative test (Kaiser Test) and On-Bead FT-IR spectroscopy were used. The structure of Glutamate-Urea-Lysine (EUK) was confirmed using LC-Mass and 1H-NMR. This novel method successfully applied to synthesize of another urea-based peptide containing a sequence of Glu-Urea-Lys(OMe)-GABA-Tyr-Tyr-GABA and the bifunctional linker hydrazinonicotinamide (HYNIC) as well.

Vitamin D3 deficiency can causes many diseases such as rickets, osteopenia and osteoporosis and increases the risk of some types of cancer. Probiotic strain of Lactobacillus plantarum, which can store fat-soluble vitamin D3 in its bulk, can reduce the effects of vitamin D3 deficiency in addition to being able to produce products with probiotic benefits..
This research was carried out in 2016. By designing the one-factor-at-a- time tests, the range of possible effective variables on vitamin D3 absorption in bacterial mass and effective factors were selected. Optimization of vitamin D3 entrapment in biomass of bacteria was performed using response surface methodology via Box-Behnken design. The high-performance liquid chromatography was employed for determination of vitamin D3 quantities.
Among the parameters affecting vitamin D3 entrapment, three factors including incubation temperature, initial vitamin D3 and sucrose concentrations were most effective. The optimal points were obtained at vitamin D3 concentration of 351723.537 IU/mL, sucrose concentration of 2.89 (g/L) and incubation temperature of 33.8 °C. The maximum value of vitamin D3 in dry cell weight of L. plantarum was 1028.5 IU/g which was consistent with the proposed statistical model.
In this study L. plantarum enriched with vitamin D3 was produced and optimized for the first time. Experimental and statistical studies confirm the accuracy and reliability of this optimization.

Designing enriched probiotic supplements may have some advantages including protection of probiotic microorganism from oxidative destruction, improving enzyme activity of the gastrointestinal tract and probably increasing half-life of micronutrient. In this study Saccharomyces cerevisiae enriched with α-tocopherol produced as an accumulator and transporter of a lipid soluble vitamin for the first time. By using one variable at the time screening studies, three independent variables were selected. Optimization of the level of dl-α-tocopherol entrapment in S. cerevisiae cells was performed by using Box-Benkhen design via design expert software. A modified quadratic polynomial model appropriately fit the data. The convex shape of three-dimensional plots reveal that we could calculate the optimal point of the response in the range of parameters.The optimum points of independent parameters to maximize the response were dl-α-tocopherol initial concentration of 7625.82µg/ml, sucrose concentration of 6.86 % w/v, and shaking speed of 137.70 rpm. Under these conditions, the maximum level of dl-α-tocopherol in dry cell weight of S. cerevisiae was 5.74µg/g. The resemblance between the R-squared and adjusted R-squared and acceptable value of C.V% revealed acceptability and accuracy of the model.

Epilepsy is a chronic disorder of the brain affecting around 50 million people in the world. Up to 30% of epileptic patients do not respond to available drugs and medical therapies. In this paper, anticonvulsant screening of 10 synthesized new derivatives of 1, 4-dihydropyridine 3, 5-dicarboxamides was performed. Anticonvulsant activity was evaluated by intravenous and intraperitoneal pentylenetetrazole and maximal electroshock induced seizures tests. Nifedipine was used as reference drug. Our pharmacological results revealing the compounds 2, 4, 5, and 6 can be effective in both absence and grandmal seizures in human. These pharmacological studies have displayed that these new dihydropyridine derivatives are capable to inhibiting seizures induced by pentylenetetrazole and maximal electroshock in mice efficiently.

A rapid, simple and reproducible HPLC method was developed and validated for the analysis of nimodipine (NM) and/or its metabolite, oxidized nimodipine (OX–NM) in rat cerebrospinal fluid (CSF) and artificial CSF. The NM and OX–NM were eluted in less than 10 min with no interferences from the endogenous CSF peaks. Analysis was carried out on a Eurospher Performance (RP-C18, 250 × 4.6 mm) column and UV detection at 236 nm. The mobile phase consisted of acetonitrile and water (70:30 v/v, respectively) with a flow rate of 1 mL/min. Limit of detection was 0.1 μg/mL for OX-NM. The calibration curve was linear over the concentration range of 0.5-10 µg/mL and analytical recovery was more than 95%. The coefficients of variation for intra-day and inter-day assays were less than 5%.

Infectious diseases are still one of the main causes of death according to the World Health Organization (WHO) reports. Plants due to their biochemical metabolites have been considered as one of the important sources for investigation in this field. Ethnobotanical and ethnopharmacological researches are considered effective in developing new anti-infectives. Sumac (Rhus coriaria L.) has been used as an anti-infective agent by ancient Iranian medical sages.
The aim of this study was to isolate bioactive agents of sumac epicarp with probable antibacterial activity.
Grounded epicarp of sumac fruit was fractionated with different solvents. The fractions were dried and subjected to antibacterial investigation. Ethyl acetate fraction showed the strongest antibacterial activity. This fraction was further investigated through TLC-bioautography which led to the isolation of two crystallized compounds. The structure of these compounds (1 and 2) was identified using spectroscopic techniques. Isolated compounds were tested for antimicrobial activities.
Compound 1 which was named 1,2-dioxo-6-hydroxycyclohexadiene-4-carboxilic acid was isolated from R. coriaria L. for the first time. It showed antibacterial activity against Staphylococcus aureus (minimum inhibitory concentration [MIC] = 0.02%). Compound 2 which was identified as gallic acid showed weak antibacterial effects on both gram-positive and gram-negative bacteria (MIC > 0.1%).
This is the first report about the chemical structures of antibacterial constituents of R. coriaria L. Previous studies have shown anti-methicillin-resistant S. aureus (MRSA) activity of sumac total extract. Compound 1 as the most effective anti-S. aureus component of sumac extract would be responsible for this activity and could be the subject matter for future investigations.

To evaluate the efficacy of different ways of communications on prevention of suicidal reattempt, we compared the efficacy of phone call versus face‑to‑face follow‑up in patients with previous attempt.

In a randomized controlled clinical trial, 55 suicide reattempters who were admitted to the poisoning emergency were divided into phone call (29 patients) and face‑to‑face (26 patients) groups randomly. They were followed at 8 occasions: 2nd and 4th weeks, and the 2nd, 3rd, 4th, 5th, 6th, and 8th months. The suicidal reattempt, suicidal thought, hope, and interest of the patients were assessed on each occasion, and the patients were guided to visit by a therapist, if needed. The findings were analyzed by Mann–Whitney, Chi‑square, Cochran, Friedman, and independent t‑tests using SPSS 20.

The status of “hope” and “interest” improved in both groups, but it showed more significant difference in the face‑to‑face group. Suicidal thoughts in both groups decreased over time, and this was more significant in the face‑to‑face group. However, we did not found any significant difference in the frequency of the suicidal reattempts between two groups.

Face‑to‑face versus phone call follow‑up of suicide attempters can significantly alleviate suicidal thoughts and improve hopes and interests