جستجوی مقالات مرتبط با کلیدواژه « زوفا » در نشریات گروه « زراعت »

Salinity stress is one of the important damaging factors for seed germination which interrupts the uptake of some nutrients by lowering soil water potential and causing ion toxicity due to salt accumulation inside the seeds. Since hyssop seeds are sensitive to salinity stress in the early stages of seed germination, it is necessary to investigate seed enhancement methods to improve seed germination in this species under saline conditions. In this research, the effects of seed priming using iron were studied on hyssop seed germination characteristics under salinity stress conditions.

This research was conducted as two separate factorial experiments in complete random design with three replications. The treatments included five levels of iron nano-chelate and micro-chelate (zero, 50, 100, 200, and 400 mg /l) and six levels of salinity (zero, 50, 100, 150, 200, and 250 mM) and two priming durations. It included 12 and 24-hours.

The results showed that with the increase in salinity, the germination characteristics declined so at 200 mM of salinity, seedling growth declined significantly and ceased completely at 250 mM level. Application of iron micro-chelate under non-stress conditions did not have any positive effect on hyssop germination, and the use of distilled water for 12 hours worked better than iron nano-chelate. No significant difference was observed among the different concentrations in micro-iron treatment. Application of nano and micro chelate at 100 and 150 mM salinity stress had a stronger effect so that nano iron chelate concentrations of 200 mg/L for 12 hours and 100 and 200 mg per liter concentrations of iron micro chelate had the highest germination, seedling growth, and seed germination in 24 hours. The amount of soluble protein and peroxidase and catalase activity increased at 50 mg/L salinity but decreased at higher salinities. Also, pretreatment of seeds with an iron micro-chelate and nano iron chelate concentration of 200 mg/liter intensified this process.

The results of this study showed that seed germination of hyssop is highly declined due to salinity stress, so the seedlings were unable to grow at a salinity concentration of 200 mg/L. Moreover, germination does not occur at 250 mg/liter salinity. Also, seed pretreatment of this plant is not justifiable under non-stress and mild stress conditions, and pretreatment with iron chelate had negative effects and was ineffective at best. However, iron chelate application at high salinity levels- especially in the nanoscale with a concentration of 200 mg/liter for 12 hours- is effective in reducing the effect of salinity.

Highlights:

1- The role of the iron element was investigated by seed pretreatment method to alleviate the negative effects of salinity stress on hyssop seed germination characteristics.
2- The nanotechnology used in the production of iron fertilizer and its effect on improving the germination characteristics of hyssop under salinity stress were investigated.

In this experiment the effect of the foliar applications of proline, yeast extract, and urea on thegrowth, yield, and quality characteristics of active substance of hyssop was investigated. This study was done in a split plot experimentalin randomized complete block design with three replications at the experimental field of Shahrekord University in 2017. The main plot was sole cropping of fenugreek, sole cropping of hyssop, and three intercropping ratios i.e. %50 fenugreek + %50 hyssop, %25 fenugreek + %75 hyssop, and %75fenugreek + %25 hyssop. The sub-plot was four levels foliar sprayings i.e. non-spraying or control and foliar sprayings of proline, yeast, and urea.The results showed that the intercropping and foliar sprayings of proline, yeast, and urea were significantly affected on some characteristics of hyssop such as plant height, number of lateral branches and the contents of chlorophyll a and b. The amount of land equivalent ratio (LER) in the intercropping was more than hyssop monoculture. In addition, LER under the foliar spraying of proline, yeast, and ureawas higher in comparison with non-foliar application.The pattern planting and foliar sprayings of proline, yeast, and urea had significant impacts on the proline content in the hyssop plants. Also, effect of monoculture of hyssop on essential oil content and yield of hyssop wassignificant; however, the foliar application of proline, yeast, and ureahad not influences on two these characteristics. In conclusion, the intercropping hyssop with legume (fenugreek) and the foliar sprayingsof proline, yeast, and urea can improve somegrowth parameters and active substance or the quantity and quality essential oilof hyssop.

To investigate the effect of iron, zinc, and manganese on morphological and phytochemical traits of hyssop, an experiment was conducted as a randomized complete block design with three replications in 2016. Treatments consisted of control, foliar application of iron, zinc, manganese, iron + zinc, iron + manganese, zinc + manganese and iron + zinc + manganese. The foliar application had a significant effect on all studied traits. Foliar application, especially in combined treatments of the above-mentioned microelements, had a positive effect on all studied traits compared with control. The highest values of plant height (49.55 cm) and biological yield (5085.7 kg/ha) were gained with foliar application of iron + zinc + manganese. The untreated control produced the lowest plant height, number of lateral branch per plant, number of flowering branches per plant and biological yield. The foliar application significantly increased the number of flowering branches per plant and biological yield compared with control. The highest total chlorophyll (1.85 mg g-1 FW) was gained by foliar application of Fe. In addition, foliar application with the combination of microelements produced the greatest total flavonoids content in plants. Results showed that simultaneous foliar application of Fe, Zn and Mn had a positive effect on the quantitative and qualitative traits of hyssop. Therefore, micronutrients could be applied as a suitable strategy to reduce chemical inputs toward sustainable agriculture and to achieve optimum biological yield in hyssop.

Intercropping can be considered as one of the ways to increase yield and sustainability of production per unit area. Also, medicinal plants play an important role in human health in the world. Many people in different countries of the world tend to use these drugs. The need for these plants is increasing due to the production of herbal medicines and cosmetics in the national and international markets. Therefore, improve quality of natural medicinal plants is an absolute necessity. In order to improvement of soil fertility and hyssop (Hyssopus Officinalis) guality by biological nitrogen fixation with bacteria is known by lentil (Lens culinaris), the experiment was conducted to evaluate the different combinations of intercropping hyssop and lentil. The experiment was performed in 2016, based on randomized complete block design with three replications in the Research Field of Imam Khomeini International University, Qazvin, Iran. The experimental treatments includes: pure culture of hyssop (A), pure culture of lentil (B), 100% hyssop + 25% lentil (C), 100% hyssop + 50% lentil (D), 100% lentil + 25% hyssop (E) and 100% lentil + 50% hyssop (F). The results of this study showed that the highest dry weight of hyssop per unit area was obtained from its pure culture (A) and 100% hyssop + 25% lentil (C). The highest content of hyssop essential oil (without statistical difference) was obtained from 100% hyssop + 25% lentil (C) and 100% hyssop + 50% lentil (D). Means comparison of hyssop essential oil yield per unit area showed that the pure culture of hyssop (A) and 100% hyssop + 25% lentil (C) had the maximum amounts without statistical difference. The yield of lentil in its solecropping treatment showed the highest value, which showed a significant difference with different intercropping treatment. The lowest yield of lentil per unit area was obtained from treatment C (25% lentil + 100% hyssop), which showed a significant difference with other treatments. Also, the results of mean comparisons showed that D treatment (100%hyssop + 50%lentil) had the highest 1000-grain weight, which was subjected to B (sole cropping of lentil) and C (25% lentil + 100% hyssop) treatments in the same statistical group. Comparison of mean values showed that sole cropping (A) and 100% lentils + 25% hyssop (E) had the highest leaf nitrogen content and lentil solecropping (B) had the highest nitrogen content in lentil seed.The comparison of Land Equivalent Ratio (LER) showed that all mixing ratios of hyssop and lentil (with the exception of 100% lentil + 50% hyssop - F) had the LER>1 which shows the lead of intercropping to pure culture. It should be noted that treatments C (100% hyssop + 25% lentil) and D (100% hyssop + 50% lentil) had the highest LER. Overall, the results showed that the intercropping of hyssop and lentil could stabilize the production by improving the use of resources. Also it could be significantly effective by reducing the consumption of chemical inputs with nitrogen fixation towards the ecological production of medicinal plants.

In the current work, the effect of magneto-priming has been studied on seed germination of hyssop (Hyssopus officinalis L.) - a medicinal plant from Lamiaceae - in normal condition and osmotic stress. Dehydration is a consequence of osmotic stress. The primed seeds with magnetic field (45, 90, 200, 250 mT) upon different times (5, 10, 20 and 30 min) were allowed to germinate and grow in Petri-dishes for 10 days under normal condition or osmotic stress due to mannitol (100, 200, 300 mM). At these experiments, rate and percentage of germination, length and dry weight of shootlet and rootlet of seedlings and seed vigor index were evaluated. Magnetic field (MF) (200mT/5min) significantly increased percent germination (%) and seed vigor index (%) at normal condition. Moreover, MF significantly increased seedling dry weight (25%) and rootlet length (36%). Under osmotic stress, MF significantly augmented seed germination percentage by 14%. It would be concluded that at normal condition, seed priming of H. officinalis with optimal MF could increase percent germination and promote rootlet growth which subsequently causes to improved growth through stronger establishment of seedlings and higher water absorbance from medium. Furthermore, MF would be effective for early growth of H. officinalis via enhancing seed germination under osmotic stress and/or water stress.

Investigation of salinity levels of 0 to 20 dS.m-1 of sodium chloride and Urmia Lake water on the Hyssopus officinalis showed that the minimum percent (13%), rate (1.4 per day), index (2) of germination and maximum amounts of reduction in germination (79%) were occurred at 20 dS.m-1, but the maximum one of percent (73%), rate (8.1 per day), index (23) of and minimum reduction of germination (6% increases) were occurred at 8 dS.m-1 of sodium chloride. The maximum percent (70%), rate (7.8 per day), index (23) of and minimum reduction of germination (2% increase) was obtained in 8 ds.m-1, but the minimum one of percent (54%), rate (6 per day), index (11) of and maximum reduction of germination (22% increase) Were obtained from 20 dS.m-1 of Urmia Lake water. Time to 50% germination, shoot and root length, ratio of shoot/root length, fresh and dry seedling weight were not measurable at the 20 dS.m-1 of sodium chloride. So, the longest (1.4 cm) and shortest (0.7 cm) root were respectively obtained from control (0 dS.m-1) and 16 dS.m-1 of sodium chloride. Despite of significance effect of sodium chloride on shoot/root length ratio, comparison of means showed any differences. Time to 50% germination was shortest (81 h) and longest (162 h) at 8 and 20 dS.m-1 of Lake water. The longest shoot (2.7 cm) and root (1.4 cm) was observed at 4 ds.m-1 and the shortest (0.6 and 0.3 cm) at 20 ds.m-1, respectively. The highest fresh (0.088 g) and dry (0.0077 g) weight of seedling were obtained from 16 dS.m-1 of Urmia Lake water, and the lowest fresh (0.064 g) and dry (0.0057 g) seedling weight were obtained from control.