The relationship between total antioxidant status with creatine phosphokinase and hydrogen peroxide in the athlete girls; influenced by acute exercise training

The research show that the relationship between antioxidant markers with inflammatory and oxidative stress markers levels changes was not clear, so the purpose of this study is investigation of relationship between total antioxidant status with creatine phosphokinase and hydrogen peroxide in the athlete girls influenced by acute exercise training This study was a semi-experimental research with a repeated measures design and 25 athlete girls within the age range of 21-24 years old volunteered to participate in the research after having expressed their consent through a consent form. Blood sample were collected in the three stages; pre of GXT (Graded exercise test) exercise test (grade: 5%, speed: 12 km/h, time: 20 minutes), immediately and 3 h after exercise test (recovery phase). Auto analyzer device was used for measurement of total antioxidant status, H2O2 and CPK level, also mixed model methods used for statically analysis. Total antioxidant status (TAS) concentration was faced with a significance increase after of exercise training (p≤ 0/031) (0.89 ± 0.16), but mixed model analysis indicated TAS levels has been reduced in 3h after exercise (p≥ 0/065) (0.88 ± 0.15). Plasma levels of hydrogen peroxide has not significantly changed after exercise (p≥ 0/255) (2.84 ± 1.38), and this increase was significant only in the recovery phase (p≤ 0/029) (3.04 ± 1.16), also CPK levels has been significance increased in both phases; after exercise and recovery phase (p1≤ 0/031, p2≤0.002) (96 ± 18 and 90 ± 19). The results of this study show that the immune system in athlete girls by increasing in antioxidants levels, can prevented the tissue injury caused from CPK H2O2 in response to acute exercise training. And Contrary to popular belief, CPK enzymes are effective role in stimulate of antioxidant response in athlete girls.