Evaluation of the Changes Pattern in Serum Levels of SDF-1α and IP-10 in Dogs Animal Model of Experimental Myocardial Infarction

Since chemokines expression is one of the most inflammatory responses to injury in the myocardium, this project was aimed to find out the pattern of expression of SDF-1 and IP-10 in dog model of experimental ischemia to extend the results of this model to clinical applications.

In this experimental study eighteen male dogs aged (6 - 12 months), were randomized in three groups. 1: infarction, Left Anterior Descending Coronary Artery (LAD) was ligated. 2: sham, received the same surgical procedure, excluding LAD ligation. 3: control, was held intact in the same condition. Blood samples were harvested 24 hours before operation, at the time of ligation, 2, 24 and 72 hours and 7 and 28 days after ligation in all groups and serum level of these chemokines was measured by means of ELISA test.

In infarction group the serum level of IP-10 was significantly increased (p ≤ 0.05) at the 2, 24 and 72 hours after MI and reached at peak after 2 hours. In this group, serum level of SDF-1 α at 24 and 72 hours and 7 and 28 days after MI was significantly increased (p ≤ 0.05). The peak of increase was seen in the 72 hours after MI.

U pregulation of angiostatic factors, such as IP-10, in the first few hours following injury may inhibit premature angiogenesis. Suppression of IP-10 synthesis during the healing phase may allow formation of the wound neovessels, whereas high plasma levels of SDF-1 α are clinically associated with start of angiogenesis activities..