Identification, differential and total count on haemocytes of Hyphantria cunea (Lep.: Arctiidae) and Glyphodes pyloalis (Lep.: Crambidae), and investigation on the effect of juvenile hormone I on these cells

Haemocytes of two lepidoptrans Hyphantria cunea (Drury) and Glyphodes pyloalis Walker were studied using light microscopy. Based on available literature, six types of haemocytes were recognized of each species: prohaemocyte, plasmatocyte, granulocyte, oenocyte, spherulocyte and adipohaemocyte. In general, haemocytes in G. pyloalis were smaller than those of H. cunea. This difference were quiet obvious in prohaemocytes and spherulocytes. Differential haemocyte counts showed the granulocytes and plasmatocytes to be the most abundant haemocytes and were recorded 27 and 23 percent for G. pyloalis, and also 28 and 36 percent for H. cunea, respectively. Total haemocyte count were also worked out that was 1800 ± 0.081 cells/mm3 for G. pyloalis and 3013.3 ± 413 cells/mm3 for H. cunea. While bringing about major structural changes to important cell types i.e. plasmatocytes and granulocytes, the juvenile hormone I (JH I) in 0.004 μL/Larva on topical application induced significant changes in total and differential counts compared to controls.