Quantification of Schooling Behavioral Responses of Rosy Barb (Puntius barbus) to Acute Decrease Temperature Fluctuations Using Computer Vision

In order to quantify of schooling behavioral responses of Rosy barb (Puntius barbus) subjected to acute decreasing temperature stress monitored by computer- vision system. Four aquaria as duplicate with a density of 30 fish per unit were used for rearing of fish in normal and stress conditions. Some geometric schooling parameters such as average location of center gravity school and distance of coordinate axels X, Y, Z and average of swim speed were used for calculating. Additionally mobility and density of fish school were evaluated by the ‘Projected Mobility Picture’ (PMP) index. Expressed indices for normal temperature condition were compared with acute stress of low temperature. Cortisol was measured from whole body with sampling 6 fish before and after stress. The results showed that schooling behavioral was changed by entering, 8◦C water, and expressed indices were different significantly. While average location of center gravity school and distance of coordinate axels X, Y, Z showed intensity fluctuations in all three directions was correlated with spreading and contracting of the school. PMP index showed an accumulation of the school in the outlet area. The average of swim speed of fish school decreased under stress conditions compared to normal conditions (P<0.05). Cortisol levels increased significantly after stress which support behavior parameters (P< 0.05).