The Synergic Effects of Atorvastatin (0/1m) and (10 m) and Extremely Low Frequency Electromagnetic Field on Angiogenesis

statins promote the proliferation and survival of endothelial cells. Angiogenesis is the formation of new blood vessels from pre-existing ones. It contributes to physiological and pathophysiological condition. In this research we used atorvastatin (0/1m) and (10m) with electromagnetic field on angiogenesis of chick embryo. in this experiment study 98 Ross fertilized eggs were randomly divided in to 7 groups as: following: control group،1)group sham-exposed،2) group treated with atorvastatin (0/1 m)، 3)group treated with atorvastatin (10m)، 4) group treated with electromagnetic field،5) group treated with both atorvastatin (0/1m) and electromagnetic field،6) group treated with both atorvastatin (10m) and electromagnetic field. In day2، a window was opened on eggs in sterile condition in days 8a gelatin sponge was placed on chorioalantoic membrane (CAM) and was soaked with 10l atorvastatin (0/1 m) and (10m) in groups (3،6) and groups (2، 5) and groups (4،5،6) were placed in 50 Gauss magnetic field for 4 hours in day 10. In day 12،CAMs were examined and photographed by research photo-stereo microscope in all case. Data were analyzed statistically by t-test and ANOVA (P<0/05). comparison between average umber and length of vessels in controls and sham-exposed didn’t show and significant differences (p>0/05). comparison between average number and length of vessels in control and group 2 and groups 3، 6 a significant increase and decrease was shown in the average number and length of vessels compared with control (p<0/05). the result of this study showed that: treated with atorvastatin 10m with electromagnetic field has an inhibitory effect on angiogenesis in CAM and was shown that electromagnetic field has an inhibitory on atorvastatin 0/1m to stimulatory effect on angiogenesis in CAM.