Chemical Assessment of Active Ingredients and Anti-oxidant Effects of Trachyspermum Copticum's Seeds harvested in Yazd Province

rachyspermum copticum as a medicinal plant has many therapeutic properties including; anti-flatulence, anti-emesis, anti- rheumatism and expectorant. The goal of this study was to identify the active ingredients and anti-oxidant effects of Trachyspermum Copticum's seeds harvested in Yazd province. The essence of the seeds was first extracted by Clevenger apparatus. The active components of the essence were then separated and identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) methods. The anti-oxidant effect was determined by DPPH (2, 2-diphenyl-1-picrylhydrazyl) test as the half maximal inhibitory concentration (IC50) and the total amount of phenolic components of the essence was quantified utilising the Follin-Ciocalteu method. The results of this study revealed that thymol (64.9%) and γ-Terpinene (11.1%) were the most prevalent components of the essence. Also, the anti-oxidant activity and the total amount of phenolic component of the essence were 0.711µg/ml-1 and 162.62 mg/g-1 resepectively. The result of this research indicated that the active ingrediebts of native Trachyspermum copticum harvested in Yazd province were much higher than the ones found in Trachyspermum copticum harvested in other places.