In vitro Culture Improvement of Five Cut Rose (Rosa hybrid L.) Cultivars

In vitro culture of plants offers high capacity for multiplication, maintenance and manipulation of modern cultivars. Five commercial cut rose cultivars in Iran (Cherry Brandy, Cool Water, Full House, Maroussia and Super Green) were evaluated in this study. Nodal stem segments (2 cm) were surface sterilized with commercial bleach (10-50%) for 20 min and 70% ethanol (v/v) for 2-5 min and cultured on MS medium. Various concentrations of 6-benzyladenine (BA) (0 -0.55 -1.11 -2.22 µM) and naphthalene acetic acid (NAA) (0- 0.054- 0.27- 0.54 µM) were used for shoot proliferation of new establishments. Two types of auxin, NAA (5.4 µM) and IBA (9.9 µM) were tested for root induction. Higher percentage of aseptic shoots was obtained by 50% commercial bleach and 5 minute of 70% ethanol pretreatment. Significant difference was observed between shoot proliferation rates (0.0- 82%) of cultivars and all cultivars had satisfactory multiplications on 2.22 µM BA and 0.27µM NAA combinations. The rate of produced root (0- 71.4%) differed between cultivars.