PARENTAGE ANALYSIS IN THE PROGENY OF TWO APPLE CULTIVARS USING MICROSATELLITE MOLECULAR MARKERS

In order to analysis the parentage of offsprings from a controlled crosses, recognizingseedlings from unwanted pollination and evaluation of homogeneity of the analyzed trees, 30 seedlings of the cross (‘Red Spur’ × ‘Golab-e- Kohanz’) and the cultivars ‘Red Spur’, ‘Golden Smoothee’, ‘Gerany smith’, ‘Golab-e-Kohanz’ and ‘Shafi Abadi’ as probable parents were evaluated with microsatellite markers. From 13 pair of microsatellite primers, CH01d018, CH03d07, CH02g01 and Col pair primers could separate actual parents of these offsprings from the other cultivars using exclusion method and likelihood based assignment method. Allele distribution among the offsprings determined their codominant mode of inhertance and no significant differences with Mendelian codominant ratio were observed using chi-square test. The results of this experiment could recognize a progeny from an unwanted cross. In each parent cultivar, between several trees used as plant materials no difference was observed using microsatellite analysis.