genetic diversity of four finger threadfin (Eleutheronema tetradactylum) population in the Persian Gulf and the Oman Sea using 28S rRNA gene Sequencing

Genetic population structure in four finger threadfin (Eleutheronema tetradactylum) was examined by using sequencing analysis in the Persian Gulf and Oman Sea. Forty one samples were collected from four locations; Khoozestan, Booshehr, Bandarabas and Systan and Baloochestan. Genomic DNA was extracted using Phenol_chloroform method. Forward and reverse primers were designed based on 28s rRNA gene in four finger threadfin. After PCR and sequences alignment, data statistical analysis was performed by Dnasp V:5, Arlequin V:3.5 and MEGA V:4 softwares. Maximum haplotype and nucleotide number and diversity were found in Bandarabas region and minimum of those indicators was recorded in Booshehr region. The highest genetic distance was between Booshehr and Bandarabas regions (0.29) and the lowest was between Booshehr and Chabahar (zero) regions, respectively. The highest and lowest sequence divergence were between Chabahar_Khoozestan (0.71) and Booshehr_Chabahar (0.05), respectively. Maximum haplotype frequencies rate were found between Bandarabas_Booshehr (12.37) and Booshehr_Khoozestan (7.95) populations (P<0.05). Maximum rate of haplotype and nucleotide diversity was estimated between Khoozestan_Booshehr regions and minimum for those indicators were recorded between Booshehr_Chabahar regions. Regarding the results it can be stated that Booshehr is an old population and has reached to the genetic stable structure, but the others have an aspect of high dynamic genetic population. So, all of them sets in an one cluster in phylogenetic tree and the out group was reset in another cluster.