Effect of Cortisol and Morphine on Ghrelin Concentration in Female Sheep With Diffrenet Diets

Ghrelin increases food intakes and body weight while weight loss and fasting is accompanied by increased levels of cortisol. The goal of this study was to determine the effects of cortisol and morphine on ghrelin concentration in ewes with different levels of energy requirements.Ten Zandi ewes were randomly divided in to two groups. The groups were fed either by 50% or 100% of energy content in diet for 10 days. After a week animals in both groups received cortisol(60 μg/kg), morphine(0. 15 mg/kg) or simultaneous injection of cortisol and morphine respectively. Blood samples were collected 2 hours after infusions.Ghrelin concentration significantly increased in the 7th day of experiment compared to first day in the %50 group. While in the group by 100% energy level, significant changes wasn’t observed in ghrelin concentration. After injection of cortisol ghrelin reduction in the both groups. Morphine increased ghrelin in both groups. After simultaneous injection of cortisol and morphine there was no significant increasing and decreasing changes in both groups.