The Anti- Proliferative and Lethal Effects of D-alpha Tocopheryl Succinate (Vitamin E Succinate) and Honey Bee Venom(BV) on Human PromyelocyteLeukemia Cell Line (HL-60)

Acute promyelocytic leukemia is the most common leukemia among adults. Nowadays vitamin E has been used for cancer treatment in research and clinical approach. Application of high concentrations of vitamin E to induce apoptosis have side effects so in this study we examined the effect of bee venom or vitamin E or both together on the induction of apoptosis in HL-60 cell line in order to increase anti-proliferative ability and reduce side effect of vitamin E. HL-60 cell line were cultured in RPMI medium and then treated with different concentrations of vitamin E and bee venom. The effect of bee venom and vitamin E on cell viability and proliferation were measured by MTT assay and cell counting trypan blue staining. Data were analyzed using one-way ANOVA test and Instate 3 software. Our results show that inhibitory effect of vitamin E increased dramatically in the presence of bee venom. On this basis we suggest that non- toxic concentration of bee venom can increase anti –proliferative activity of vitamin E on HL-60 cancer cells. Our result show that non-toxic concentration of BV can increase inhibitory effects of vitamin E and it make hope that BV can increases the anti-proliferative potential of vitamins in non-toxicconcentration and concentration withoutside effectsin the future.