POU1F1 gene polymorphism of Makuyi sheep breed and its association with growth- related traits using by PCR-SSCP

POU1F1 (also named PIT-1 or GHF-1) as a member of the POU family of transcription factors، is mainly expressed in the pituitary and has positive regulation on growth hormone، prolactin، thyroid-stimulating hormone β، POU1F1 itself and growth hormone releasing hormone receptor genes. In order to investigate POU1F1 gene polymorphism of Makuyi sheep breed، after extraction of DNA with salting out method from blood samples of 95 males and females belonging to this breed from breeding center of Maku، the polymerase chain reaction was used to amplify 295 bp DNA fragment (a part of intron 2، exon 3 and a part of intron 3) with a pair of designed specific primers. The single stranded conformation polymorphism (SSCP) patterns of PCR products were studied using 6% polyacrylamide gel and silver staining method. Finally 4 band patterns AA، AB، CC and CD were obtained with frequencies of 0. 45، 0. 073، 0. 44 and 0. 037، respectively. The frequency of A، B، C and D alleles was calculated as 0. 4895، 0. 0368، 0. 4579 and 0. 0158، respectively. Gene diversity was 55. 2%. The results indicated that Makuyi sheep population was not in Hardy-Weinberg equilibrium (P<0. 05). Significant (P<0. 05) statistical relationships between polymorphism of POU1F1 gene and body weights at 9 (W9) and 12 (W12) months were found. The CC genotype was associated with highest W9 and W12 (33. 8 and 44. 75 Kg، respectively) than AA genotype.