Changes of Serum Inflammatory Markers after Gamma-Secretase Inhibitor Administration Mice

Notch signaling is involved in physiological and pathological processes and gamma-secretase inhibitors can suppress it. The aim of this study was to evaluate the effect of DAPT، a gamma secretase inhibitor، on serum inflammatory markers in mice. 24 C57BL6 mice (age: 5 weeks old) were randomly divided into control and obese groups. Obese group received high-fat diet (60% fat، 20% protein، and 20% carbohydrate) for 20 weeks. Control group received standard diet during this time. Then، each group was divided into receiving and non-receiving DAPT (50mg/kg; Subcutaneous). After 14 days، blood samples were taken for measurements of serum high-sensitive C-reactive protein (hs-CRP) and interlukin-6 (IL-6). There was no significant difference in serum hs-CRP concentration between control and obese groups (P > 0. 05). Administration of DAPT did not alter serum hs-CRP level in both groups (P > 0. 05). Serum Il-6 was significantly higher in obese animals compare to controls (P < 0. 05) and administration of DAPT reduced serum IL-6 in control and obese animals (P < 0. 05). DAPT، as a gamma secretase inhibitor، could affect several physiological and pathological vascular changes by alteration of serum inflammatory markers.