Determination of Triton X-100 in water samples using dispersive liquid-liquid microextraction and HPLC with the aid of response surface methodology
Author(s):
Abstract:
An improved method for the determination of Triton X-100 in water samples has been developed using dispersive liquid–liquid micro extraction (DLLME) prior to HPLC analysis. The volume of extraction solvent (CHCl3)، disperser solvent (acetone) and sample solution، pH، ionic strength، and extraction time were selected as interested variables in DLLME process. Firstly، a screening experiment، Taghochi two-level orthogonal array، was employed to identify the important factors. Then the elucidation and optimization relationship between the response and the important factors was investigated by response surface methodology (RSM). The central composite design as the most popular of the many classes of RSM designs was used. The optimum experimental conditions found from this statistical evaluation were included: sample volume: 10. 0 mL، volume of extraction solvent (chloroform): 137. 7 µL، volume of disperser solvent (acetone): 0. 6 mL، centrifugation time: 5. 0 min، pH: 6. 2 (natural pH)، 2. 5% (w/v) NaCl and extraction time: 6. 0 min. Under the optimum conditions، the preconcentration factor of 120 was achieved. Calibration graph was linear in the range of 0. 01-100. 0 mg L−1 with correlation coefficient of 0. 9961. The Limit of detection (LOD) of 3. 0 µg L-1 was obtained for Triton X-100 determination
Keywords:
DLLME , Triton X , 100 , Experimental design , HPLC
Language:
Persian
Published:
Journal of Applied Chemistry Today, Volume:7 Issue: 23, 2012
Pages:
63 to 70
magiran.com/p1146790
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!