Isolation the cellulolitic microorganisms from soil and optimization FPA by suitable strain

In this research، we studied isolation of the cellulolitic fungi from the persimmon tree، grapevine، pomegranate tree and walnut soils. Among of them، persimmon soil was selected because of maximum cellulolitic fungi. Seven fungi werw isolated from persimmon soil، which three of them had suitable cellulose activity and they were identificated by 18S rRNA and named: Aspergillus niger MZM 89-a2، Penicillium decumbens ZHE 89-p3، Penicillium decumbens MMH 89-p1. Cellulase activities of these fungi were respectively (U/d): FPA 3. 1671، 3. 5740، 3. 1812 and Avicelase 1. 6605، 0. 3869، 1. 1451 and CMCase 2. 950، 0. 2644، 0. 4604. Response surface activity (RSM) was studied to evaluate the effects of temperature، moisture content and particle size for FPA by Aspergillus niger MZM 89-a2. The optimum FPA was in temperature، moisture content and particle size respectively: 28. 49ْC، 66. 62٪ and 1. 5-2 (mm). The maximum predicted FPA was 4. 35 (U/g) and obtained FPA under this condition was 4. 42 (U/g)، which indicates the efficacy of the model for prediction of FPA activity under different conditions of the medium.