Optimization of Culture Conditions and Medium Components for Carboxymethyl Cellulase (CMCase) Production by a Novel Basidiomycete Strain Peniophora sp. NDVN01

Cellulytic enzymes including carboxymethyl cellulases play the key role in hydrolysis of cellulose, a huge organic carbon reservoir on earth, into monomeric sugars and their eventual conversion into valuable chemicals and energy sources.. In this study, we described the identification of a basidiomycete isolate NDVN01 and optimization of culture conditions and medium components for CMCase production by this strain under liquid state fermentation. The CMCase was estimated as 32 - 33 kDa on a native Polyacrylamide gel electrophoresis (PAGE).. We used 5 basidiomycetes for screening CMCase production, internal transcribed spacer (ITS) sequence analysis in combination with morphology for strain identification, and liquid state fermentation for optimization of CMCase production.. The maximum CMCase production by Peniophora sp. NDVN01 was obtained at 28°C, with the initial medium pH of 7 and within 120 hours of cultivation in the optimum medium containing 80% (v/v) of potato infusion, 0.6% (w/v) straw rice as additional carbon source and 0.2% (w/v) ammonium hydrogen phosphate as an additional nitrogen source, and 0.5% (w/v) pulp as inducer.. Under optimal conditions, Peniophora sp. NDVN01 produced 24.65 ± 0.37 units of CMCase per mL of culture supernatant, which was 8.6 times higher than the amount (2.87 ± 0.28 U.mL -1) before optimization..