Growth Kinetic and Carotenoids Production by porobolomyces Ruberimus H110 Using Ammonium Sulfacte in Batch Reactor

At this study, growth and production of carotenoid (torularhodin and β- carotene) by a new isolated strain (Sporobolomyces ruberimus H110) were studied using technical glycerol and ammonium sulfate as souces of carbon and nitrogen, respectively. Experiment was carried out in a 3L reactor batch in which glycerol concentration was kept constant but three different concentration of ammonium sulfacte (3, 20 and 30 g/l) were used at 23ºC and pH=6. The maximum yield of toularhodin (2.70mg/l) and β- carotene (0.10 mg/l) was obtained using 20 g/l ammonium sulfate. The highest amount of μmax (0.096 h-1) obtained with supplying of 30 g/l ammonium sulfate. The optimum production (growth yield as a function of glycerol consumption for different intial sulfate ammonium concentrations) was 0.52±0.01 g/g in all of the experiments.