Cytogenetic analysis of Rosa damascena Mill. from different regions of Iran

Rosa damascena Mill. is one of the valuable species with a long history in Iran and some other countries. In order to investigate cytogenetic variation in Rosa damascena، meristemic zone of 14 accessions were used. The research was conducted using a completely randomized design. Image analysis system and Micro-measure software were used to karyotype providing and cytogenetic parameters measurement. Data were collected and analyzed using a completely randomized design with three replications. Results showed significant differences (P<0. 01) among accessions for some parameters، such as short arm (SA)، long arm (LA)، arm ratio (AR)، Intra asymmetry chromosomal index (A1) and total form percentage (TF%). Using principal components analysis، the first three independent components accounted over 98% of variation. The first principal component indicated that short arm (SA)، arm ratio (AR)، intra symmetry chromosomal index (A1) and total form percentage (TF%) are appropriate parameters to classify accessions with 51% of total variation. Long arm percentage and Short arm percentage were important traits in second component at 24% level. Long arm and Total length (TL) were important traits in third component at 22% level. Cluster analysis based on cytogenetic parameters، grouped the accessions into 4 categories. The most far apart accessions were Isfahan9 and Ilam1 and the most near ones، Gilan 1 and Isfahan 7. It is necessary to notify distribution of accessions based on the first three component scores were in agreement with cluster analysis.