Genetic Diversity among Some Iranian Tulip Species Using Molecular Marker RAPD

Tulip (Tulipa L.) is one of the most important bulbous plant in the world that has a great spread over the country. This genus belongs to the lily family (Liliaceae) and contains almost one hundred species, from which 19 species exist in Iran. In this study genetic diversity among 18 Iranian tulip landraces of 6 species and 3 cultivars from T. gesneriana were investigated with RAPD molecular marker. From 14 used primers 69 bands were produced from which 67 bands showed polymorphic. All of the primers except BC14 showed large polymorphism to 100% and only BC14 showed 50% of polymorphism. Largest resolving power (Rp) was 4.28 in 13th primer. According to the results obtained from similarity matrix, the greatest similarity between T. gesneriana1 and T. gesneriana2 was 90% and the smallest similarity between T. montana3 and T. systola1 was 0.06%. By cluster analysis of obtained data with UPGMA method and Jaccard similarity coefficient with 35% of similarity, divided genotypes into 5 groups. Percentage of diversity was 57% between species and 43% within species; which shows a great inter and intraspecies variety among the studied genotypes. Such a great diversity in the modifying processes can be used as a useful modifying device.