Isolation and Molecular Detection of a HexadecaneDegradingBacterium from Compost and Evaluation of its Performance in the Removal of Hexadecane
Author(s):
Abstract:
Introduction
Petroleum hydrocarbons are the most frequent environmental pollutants. Contamination of soil and groundwater by diesel released from underground storage tanks is an important and extensive environmental problem in Iran. The aim of this study was to isolate and molecular identification of n-hexadecane-degrading bacteria from compost. Methods
Hexadecane، C16H34، used as a model contaminant of diesel oil. New n-hexadecane degrading bacteria was isolated from compost by using enrichments on n-hexadecane. Then isolated bacterium was identified by PCR with 16S rDNA method. Then n-hexadecane degradation was tested by isolated bacteria. Finally response of strain to different salinity concentrations (0، 1، 2. 5 and 5) was determined. Results
Based on morphology، biochemical tests، 16S rDNA sequence analysis and phylogenetic characteristics، the isolated bacteria were identified as Serratia marcescens. After 33 days، n- Hexadecane concentration decreased from 90 mg to 32. 84 mg (63. 51%) by isolated bacteria. In addition، the results showed that isolated bacteria can grow on 5% salinity. Conclusion
The results of this study show that Serratia marcescens can be used in remediation of petroleum products especially diesel oil in tropical area and semisalinity of soils in Iran. Our findings indicate that Serratia marcescens is a halotolerant microorganism.Keywords:
Isolation , Diesel oil , Hexadecane , Serratia marcescens , PCR , 16S rDNA
Language:
Persian
Published:
Tolooe Behdasht, Volume:12 Issue: 4, 2014
Pages:
167 to 178
magiran.com/p1242012
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!