Investigation effect of kind and concentration of sodium alginate encapsulation in germination and growth of alfalfa artificial seed.
Author(s):
Abstract:
The alginates are most effective jelling agents in preparation of encapsulated somatic embryos in artificial seed production technique. There are different forms and viscosities of alginates. The study was aimed to investigate alginate type effect (sodium alginate LF and LB) and its concentrations (0.5, 1, 2, 3 and 4 % with MS basal medium as synthetic endosperm) on germination and growth of alfalfa encapsulated somatic embryos. Rooting and germination percentage of encapsulated somatic embryos in both in vitro (MS medium) and ex vitro (in pots containing peat moss) conditions were tested. There were significant differences between alginate types and different concentrations (p<1%). The best type and concentration of alginate with highest percent of germination of encapsulated somatic embryos in both in vitro and ex vitro conditions was LF type with 2% concentration. In this study cultivation bed of artificial seeds affected germination and development of somatic embryos to plantlets, significantly. The highest percent of germination (84/5%) were tested in vitro condition.
Keywords:
Language:
Persian
Published:
Journal of Applied Crop Research, Volume:26 Issue: 101, 2014
Page:
48
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