Assessment of Effect of Hydro-Alcoholic Extract of Scrophularia Striata on Burn Healing in Rat

Scrophularia Striata has been used to treat inflammation, ear and eye infection, skin burn and infectious wounds. This study investigated the effects of hydro-alcoholic extract of Scrophularia striata on second-degree burn. In this experimental study, 21 male Wistar rats (250-300 g) were selected. Then animals randomly divided into control and 3 treatment groups which received olive oil as vehicle or Scrophularia extract with the doses of 2.5 and 5 percent for 21 days. The perimeter and area of the wounds were measured exactly every three days. Finally, tissues samples were collected from burned area for further histological study. Comparison of wound area showed in 5% dose on 6th day (1447.77±41.73), 15th day (885.74±159.35), 18th day (556.52±122.44) (p<0.05) and 21st day (293.12±58.06) (p<0.001) and in 2.5% on 18th day (664.66±123.35), 21st day (398.94±56.00) (p<0.05) significantly different from the control group on 18th (891.98±73.52), on 21st (538.13±55.70) and olive oil group on18th (756.93±96.31) and 21st day (480.69±43.34). Comparison of wound environments showed in 5% dose on 18th day (105.24±13.14) (p<0.01), 21st day (75.06±8.61) (p<0.05) significantly different from the control group on18th day (136.65±4.62), 21st day (104.47±4.86) and olive oil group on 18th day(122.07±12.73), on 21st day (100.76±11.04). Histological study showed ulcer granulation tissue, blood vessels, exudate and collagen significantly increased in both 2.5% and 5% groups. Muscle involvement was significantly decreased in 5% group. The results showed that hydro-alcoholic extract of Scrophularia Striata are effective for burn healing and dose of 5% more effective than 2.5%.