Genetic diversity of Oriental sole (Euryglossa orientalis) using microsatellite marker in the Persian Gulf (Khuzestan and Bushehr)

The aim of this study was to analysis the population genetic structure of Eurygloss orientalis based on microsatellite markers. For these purpose 58 samples of Eurygloss orientalis from two regions of Persian Gulf (Khuzestan and Bushehr) were collected. DNA was extracted and using 6 pairs of microsatellite primers. Polymerase chain reaction (PCR) was conducted out of 6 microsatellite primers and results shows that 2 loci were polymorphic. Analysis revealed that the average number of observed and effective alleles in Khuzestan and Bushehr were 14.5, 10.708 and 13.5, 9.511 respectively. The mean observed and expected heterozygosity was 0.837 and 0.889 respectively. There are no significant deviations from HWE. The Fst value between populations was 0.025. The highest genetic distance between Khuzestan and Bushehr was 0.13 and the lowest genetic similarity was 0.733. The Fst value suggested no genetic differences between populations.