Study of Cryopreservation in Rapeseed (Brassica napus L.) Seeds by Vitrification Method

In this experiment، cryopreservation of rapeseed seeds (Milena and ARC-2 cultivars) via vitrification method was carried out using two protective solutions، PVS2 and PVS3 at 5 treatment times (20، 40، 60، 80 and 100 min). The experiment was conducted as a factorial based on completely Randomized Design with three replications. The results showed significant differences (p<0. 01) between different treatment times، genotypes and interactions of these two factor for germination percentage. Also، interactions of treatment times and protective solutions were significant (p<0. 05) for germination percentage. Treatment of rapeseed seeds (Milena cultivar) with protective solution for 100 min showed the highest germination compared with to other treatments. Also، treatment of rapeseed seeds with PVS2 solution for 100 min and with PVS3 for 20 and 60 min showed the highest germination percentage، respectively، compared to other treatment. Concerning the trait of root length، the treatment time and genotype and their interaction showed a significant differences (p<0. 01). The interaction of genotype and protective solution and also triple interaction of three factors showed significant effect (p< 0. 05) on root length of rapeseed seeds. So، treatment of rapeseed seeds with PVS3 solution for 20 min produced the highest mean for root length. Different times of treatment and also، the triple interactions of genotype، kind of solution and time of treatment showed significant effects (p<0. 01) on shoot length of rapeseed seeds. Treatment of rapeseed seeds (cultivar of ARC-2) with PVS3 solution created the highest shoot length compared to other treatments.