In Vitro Propagation of Pomegranate (Punica granatum) cv. Rabab Neyriz Using Nodal Segments

In this study، an efficient in vitro propagation method is described for Punica granatum cultivar ‘Rabab Neyriz’. The influence of two basal medium، WPM and MS، and different plant growth regulators was investigated on micropropagation of this cultivar. For proliferation stage، media supplemented with different concentrations (2. 3، 4. 7، 9. 2 and 18. 4 μM) of kinetin along with 0. 54 μM NAA was used. On base of proliferation، WPM proved to be more efficient medium compared to MS. The best concentration of kinetin was 9. 2 μM resulting in the highest shoot length (3. 93 cm)، leaf number (10. 71) and node number (4). Half-strength WPM medium supplemented with 5. 4 μM NAA was most effective for rooting of shoots. Rooted plantlets were successfully acclimatized and transferred into soil. The micropropagated plants were morphologically uniform and exhibited similar growth characteristics and vegetative morphology to the mother plants.