A Study on Toxic Effects of Diazinon on the Caspian Kutum (Rutilus Frisii Kutum) Testis Using in Vitro Tissue Culture

Caspian kutum is one of the most commercial fishes that migrates for spawning from the Caspian Sea to the rivers each year. Consequently، it can be exposed to several agricultural and industrial pollutants. In this study، to estimate the fish spermatogenesis in polluted waters، the direct effect of diazinon were investigated using in vitro testis culture of the Caspian kutum with 10، 100 and 200 µg/ ml concentrations for 3 and 6 days. With increase of diazinon concentrations and exposed time، harmful effects of diazinon on spermatogenesis and testis structure increased. The inhibition of spermatogenesis and necrotic germ cells were more visible in 200 µg/ ml in 6th day. Since، appropriate quality and quantity of gametes can verify the successful reproduction in fish، water pollution over consecutive years can be a serious threat on kutum populations