The Effect of Nicotine on the Serum Level of Glucagon and Glucose in Adult Male Rats

Nicotine is an alkaloid that is used by millions of people around the world through smoking and Effectsonthebody، especially the endocrine system. On the other hand، millions of people in the world suffer from diabetes. Hence، this study investigated the effects of nicotine on serum levels of the hormone glucagon and glucose was performed in adult male rats. Thisstudyisanexperimental study. In this research، 50 male mature rats with an averageweight of220-250 grams were enrolled as the experiment، witness and control groups. The experimental group was divided into 3 subgroups and 0. 5، 1 and 1. 5 mg per kg of nicotine was injectedintraperitoneally for 5 days. Around the sametimeevery day1 ml Physiological serum was given to the witness group، but the control group did not receive anything. At the end of 6 days، blood sample was taken from the heart ventral areas and the Serum levelsof glucagonhormone were measured using radioimmunoassay (RIA) method and the commercial kits. The results were evaluated using one-way ANOVA. According to the results of this study، Nicotineatalldosessignificantly increasedserum levels ofthe hormoneglucagon and glucose andthedosageincreased، the rateofincreaseishigher (P<0. 01). Theresults ofthis studycanbeconcluded thatnicotine whichstimulateglucagonsecretion.