Effect of Aluminum Toxicity on Inducing of Apoptosis in Cell Suspension of Rice (Oryza sativa) Khazar and Tarom Cultivars

In this study the effect of AlCl3 toxicity on apoptosis in cells suspension of two rice cultivars Khazar, Tarom were investigated. In present research cells suspension of two rice cultivars (Khazar and Tarom) grew up in liquid Murashig and Skoog medium within a period of 3 weeks. Then cells were treated with different concentrations (0, 40, 80 and 120 µmolL-1) of AlCl3 for 56 hours. Biochemical changes of DNA by gel electrophoresis and TUNEL test and morphological changes by Hoechst and propidium iodide (PI) staining were investigated. Results of gel electrophoresis and TUNEL test showed DNA damage in these cultivars. Results of Hoechst and PI dying also showed morphological changes such as: condensation of nucleus, membrane damage and shrinkage of cytoplasm. AlCl3 induced nuclear DNA damage and also morphological change in two cultivars of rice especially Tarom by increasing concentration after 56 hours of treats.