Neuroprotective effects of exercise with hydroalcoholic extraction of Eriobotrya japonica on MANF in the Brainstem of parkinson's rats

Eriobotrya japonica flower extract (EJFE) has abundant amount of phenolic compounds. This study was aimed to examine protective effects of 12 weeks of voluntary exercise on a running wheel and EJFE on intraventricular 6-hydroxydopamine-induced lesion on MANF in the Brainstem of parkinson’s rats. In this laboratory experimental study, fifty rats were divided into six groups: basic, control parkinson, training, training-parkinson, extract-parkinson and training-parkinson-extract. Training groups were housed in individual cages with attached running wheels and extracts groups during study period received 200 mg/kg extract intraperitoneally in twelve weeks three times per week. To induce parkinson, injection by 6-hydroxy‌dopamine solution (6-OHDA) (dissolved in saline) was administered into intracerebrovenricular (ICV) by a stereotaxic apparatus. MANF levels in the Brainstem were measured by ELISA. Voluntary wheel running and extract significantly prevent decreasing MANF levels in parkinson rats (P=0.001). MANF levels in training group increase and MANF levels in control parkinson decreased, but in training and extract group didn’t prevent decrease levels MANF in the Brainstem of parkinson’s rats (P=0.169). The results of this research showed that pre-treatment with voluntary exercise and hydroalcoholic extraction of Eriobotrya japonica may be increased protective against 6-OHDA toxicity and have protective role against parkinson disease.