The comparison between hydroethanolic extraction of pistacia Atlantica and Suzin effects on growth inhibition of K562 cell line in Vitro condition

Leukemiais a malignant and progressive disease of the Hematopoietic tissues of the body. Thepistaciaatlanticatreebase, the geographic inlarge areas ofthe Mediterraneanand theMiddle Eastisgrowing. K562Cell class is considered as laboratory model of chronic phase of human CML.wecompared thegrowthinhibitoryeffectsSUZIN asa chemicalcompared withpistaciaatlanticaas acombined Zn plantantioxidantcapacityinreducingcancer hasbeen studied. Pistachio nutaround Kermanwas collected.then they were dried in room temperature and extraction was performed for48 hours by maceration method.K562 Cell class wasincubatedinmediumRPMI-1640fortifiedwith10%(v/v)FBSand50%Streptomycin-Penicillin. Cytotoxiceffect of hydro- ethanolic extract ofpistaciaatlanticaagainst cancer cells K562 was evaluated in three interval by MTTmethod. Light absorbance by Eliza device was measured in wavelength 540nm. Statistical analysis was performed using SPSS15software and ANOVA test. Pistaciaatlanticain24h and inconcentration100μg/ml andSuzin in48h and in 12.5μg/ml,72 h and in 50μg/ml induced growth inhibitionhalf of thecellswereK562.results obtained from changes in cell morphology influenced by hydro-ethanolic extract ofpistaciaatlanticaand SUZINsuggest abnormal transformation of cells that probably represents apoptosis and necrosis. Time and concentration against cytotoxiceffect of Pistaciaatlanticahave the combined effect. Whileiron supplementation,alonetimeisdue. Special concentration of pistaciaatlanticahaving high antioxidant capacitywith theSuzincan be considered as a potential target for inhibitingK562cells in treatment of blood cancer.