Effect of MCP on angiogenesis in HUVEC cells and Human Prostate cancer cell line DU145

Pectins are polysaccharide rich in galactoside residues which bind to receptor Galactin-3. It has been shown pectins induce apoptosis and inhibit metastasis in tumurigenic cell via Galactin-3. In the present study، the effect of Modified Citrus Pectin (MCP) on prostate cancer cells، DU- 145 is under investigation. The citrus pectin was modified with PH and temperature alternation. Its effect on DU145 and HUVEC cells viability was studied. Thus MCP effect on angiogenesis was studied via the treatment of DU145 media on user cells. This goal، the tube formation was followed with phase contrast microscopy in a 48h period. Finding the effect of MCP on the cells migration was investigated. Our results showed that MCP is capable of decreasing DU145 viability. Indeed، this material can inhibit angiogenesis which it may be due to reduction of angiogenic factors such as VEGF in DU145 cells. Beside these، MCP showed a decreasing effect on cells migration in HUVEC cells.