Construction of plant expression cassette for production of chimeric protein containing PGIP1 and PGIP2

Polygalacturonase inhibiting proteins (PGIPs) selectively inhibit the polygalacturonases (PGs) secreted by the invading plant pathogenic fungi. The PGIPs display the differential inhibiting towards the PGs from different fungi، also towards the different isoforms of the PGs originating from the specific pathogen. In this study an expression cassette was constructed to produce a chimeric protein containing PGIP1 and PGIP2 as a fused protein using three alanine as linker. The chimeric gene is under the control of CaMV 35S promoter and NOS as terminator. Pgip1 and pgip2 genes have been amplified from Naz cultivar of bean. All the intermediate and final constructs were confirmed by PCR and restriction digestion patterns. In addition، the final construct also was confirmed by sequencing. The Chimeric construct from this study can be used to produce transgenic plants resistant to fungal pathogenes.