Evaluation of Inhibitory Effect of Dentin on the Antimicrobial Effect of Carvacrol and Sodium Hypochlorite on Euterococcus Faecalis: An In Vitro Study

different effects of disinfectants in vitro and in vivo conditions have been attributed to the inhibitory effect of dentin. The purpose of this study was in vitro evaluation of inhibitory effect of dentin on the antimicrobial property of Carvacrol and sodium hypochlorite on euterococcus faecalis. In this experimental study, an enterococcus faecalis suspension of 1.5×108 cells per militer was prepared. Fifty microliter of antimicrobial solution (sodium hypochlorite 2.5% and 1% and Carvacrol 0.6% and 0.3%) were incubated at 37°C with 28 mg dentin component (dentine powder, organic dentin component, mineral dentin component) in 50 microliter water for 1 h before adding 50 microliter of the bacterial suspension. Ten microliter samples for bacterial culturing were taken from the suspension 5min, 1 and 24 h after adding the bacteria. Serial 10-fold dilutions were made of the samples and 10 microliter of dilutions was cultured on BHI plates for 24 h at 37°C. After incubation period, colonies were counted by one person by visual observation method. Data were analyzed by Kruskal-Wallis and Mann-Withney tests (α=0.05). Dentin components, antimicrobial solutions and time, all three affected the final results. The inhibitory effect of dentin components on antimicrobial property of Carvacrol was significantly greater than Sodium hypochlorite (P<0.001). Antimicrobial effect of sodium hypochlorite is preserved at presence of dentin, but dentin inhibitory effect on Carvacrol antimicrobial property is observed after 1 h.